利用分子标记评估中国对虾放流效果分析-evaluation of the releasing effect of penaeus chinensis by molecular markers.docxVIP

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利用分子标记评估中国对虾放流效果分析-evaluation of the releasing effect of penaeus chinensis by molecular markers.docx

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利用分子标记评估中国对虾放流效果分析-evaluation of the releasing effect of penaeus chinensis by molecular markers

186/300，三个家系的识别结果为 34/100、60/100 和 92/100。分子标记的检出率要大于物理标记的检出率，对此我们再利用高温组检测以增加其排除概率，结果发现并不能排除多检出的个体分属正确的各家系。因此从一定程度上说明了分子标志较物理标志的优越性。此外，本研究还对模拟放流中投放的个体和混合背景群体进行了遗传结构上的分析以及“内标”个体在投放数量的比例进行了研究。发现在标志个体掺入前后，一个世代上的期望杂合度并没有显著性差异（p0.05）；在进行增殖放流过程中， “内标”家系的选择是比较重要的，应选择与放流群体各种指标相近的个体作为 “内标”家系，这样才能更精确的评估放流效果。关键词：中国对虾，增殖放流评估，微卫星，多重 PCREvaluation of Releasing Studies in Chinese shrimp (Fenneropenaeus chinensis) Using Molecular MarkerABSTRACTChinese shrimp (Fenneropenaeus chinensis), as an annual large economic shrimp and one of the important fisheries resource, chiefly distributed in the Yellow Sea and the Bohai Sea.To the 1980s, its resources have been depleted as the influence of overfishing, water pollution and engineering construction. Fishery resources enhancement is an effective method on the ecological environment of waters repair and fishery resources resume. The production of enhancement and the releasing of transplant of Chinese shrimp were developed at the early 1980s in China, the economic benefits and social benefits were gained as this work is taken. However, a kind of method about the evaluation of resources enhancement in Chinese shrimp has being lacked by the reason of releasing marker. The molecular marker is used for discuss the new evaluation method of resources enhancement effect, as the aim of providescientificbasis in the enhancement and the releasing of Chinese shrimp. The results as follows:Microsatellite marker is a kind of molecular marker that codominance in genetics, Mendel genetic law followed. So five loci, FCKR002, FCKR005, FCKR007, FCKR009 and FCKR013, rich in polymorphism and selected from the microsatellite sequences of independently developed Chinese shrimp, combine the three existing microsatellite loci of high polymorphism EN0033, RS0622 and RS1101, to form two groups of quadruple PCR reaction systems with the primers inside the groups marked with fluorescence labeling of 6-FAM, VIC, NED and PET. Two groups of microsatellite quadruple PC