利用改进最小表达框技术获得抗旱转基因小麦-obtaining drought-resistant transgenic wheat by using improved minimum expression frame technology.docxVIP

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利用改进最小表达框技术获得抗旱转基因小麦-obtaining drought-resistant transgenic wheat by using improved minimum expression frame technology.docx

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利用改进最小表达框技术获得抗旱转基因小麦-obtaining drought-resistant transgenic wheat by using improved minimum expression frame technology

exogenous gene GmDREB3 can be expression at the transcriptional level, further by real-time PCR analysis of transgenic wheat’s copy number, the results show that the positive plants with low copy number. These results suggest that add the SAR both ends of the minimal expression cassette sequence can significantly improve the integrity and stability of the conversion fragments, to reduce the integration of the target gene copy number.To determine the different receptor the expression level of the target gene, and trans GmDREB3 to different receptor such as Jimai 22, Shi 366, ShiB074056and Shi15 receptorwheat, and the positive rates were as follows: 0.53%，0.80%，0.99% and 0.31%, indicatingthat the ShiB074056 receptor get the highest conversion efficiency of different receptors, it’s indicating that the different receptor wheats also affect the expression level of minimal expression cassette with SAR.After the turn the gene GmDREB1 materials receptor Jimai 20 MG3a, the MG7 and MG14; Shi 4185 receptor transgenic GmDREB3 materials of the MG2-3 and the MG3-9; and JiMai 19-receptor transgenic GmDREB3 of drought physiological indexes in materials MG30-5 POD and Pro content measurement, and the yield monitor, the results show that the MG3-9, whether under the dryland treatment or water treatment conditions, POD activity and Pro content is superior so receptors and other transgenic lines; Yield performance 487.4 Kg/666.7m2 in the than in the control varieties yield 13.08% to 38.11%, ranking this first of all tested strains.Keywords: SAR sequence; Soybean DREB gene; GUS reporter gene; Biolistic transformation method; Wheat; Pro; POD; Yield AnalysisDirected by： Prof．MA Qing and Prof．MA Youzhi Applicant for Doctor degree：SU Ruibo(Crop Genetic Breeding)( College of Agriculture. Inner Mongolia Agricultural University. Hohhot 010018. China)目录1引言 ................................................................ 11.1小麦基因转化方法 ................................................ 11.1.1花粉管通道