罹病茎瘤芥pr-pr of tumorous stem mustard.docxVIP

ABSTRACTPlasmodiophora brassicae Woronin is an obligate parasite that causes clubroot, an important disease of crucifers worldwide. the yield of Brassica juncea var. tumida Tsen has been seriously affected by clubroot disease, which has caused great economic losses. Now the reaserch of clubroot only focused on the preliminary study on disease prevention and occurrence regularity.Therefore,the study of the interaction between pathogen and host plant,exploring the mechanism of plant resistance to pathogens become necessary basic work for effective prevention. PRs(Pathogenesis related proteins,PRP/PRs) is a number of proteins that were induced by pathogen attacks and associated with the course of disease,some of them are resistance proteins.The PRs reported so far can be grouped into 17 independent families.The PR4 family consists of primarily acidic proteins with low molecular weight of 13–20 kD. Members of this family have been identi?ed in both monocotyledonous and dicotyledonous plants. Existing research shows PR4 proteins have potent antifungal activity against a wide variety of pathogens.in this study, we present the isolation of a PR gene from B. juncea var. tumida Tsen.Using bioinformatics methonds analysis its characteristic and detacting the expression level in course of clubroot disease and after innoculated with salicylic acid, Methy-jasmonic acid, ethephon, abscisic acid,laying the foundation for future prevention of clubroot.The main results are as follows:According to the reported PR4 gene design the primers,the full-length (584bp) BjPR4 was obtained by RACE approaches.The largest open reading frame (ORF) is 423 bp,respectively. The predicted ORF encodes a 140 amino acid polypeptide with a calculated molecular weight of 15.5 kDa and isoelectric point of 8.13. Homology searches against GenBank database showed that BjPR4 has a high level of similarity to the plant proteins of PR4 group,so BjPR4 is one of PR4 family.Therefore, the sequence information has b