六白菖砂汤对家兔颈椎病模型外周血清中igf-1及tnf-α的影响-effects of liu bai chang sha tang on igf - 1 and tnf - α in peripheral serum of rabbit cervical spondylosis model.docxVIP

摘要目的：观察六白菖砂汤对家兔颈椎病模型外周血清中IGF-1、TNF-α的影响，以观察该方治疗颈椎病的疗效。方法：将40只成年家兔随机分为5组，空白组，颈椎病模型对照组、颈复康对照组、六白菖砂汤高剂量组及六白菖砂汤低剂量组，每组8只。采用蹲坐低头位制作颈椎病动物模型，试验中观察动物皮毛、体重、活动情况，实验开始后4周、12周所有动物拍摄颈椎正侧位X线片并用双能X线骨密度仪测量颈椎骨密度，12周时，取椎间盘做病理，取椎体检测离体骨密度，采用放射免疫法（RIA）检测外周血清中IGF-1、TNF-α因子的含量并观测。结果：颈椎病模型组和六白菖砂汤低剂量组在皮毛、体重、活动情况等方面表现相似，骨密度下降，椎间盘病理改变相似；六白菖砂汤高剂量组和颈复康对照组上述指标与正常对照组相似；六白菖砂粒剂高剂量组中IGF-1水平明显升高，与空白对照组、颈椎病模型对照组和六白菖砂粒剂低剂量组相比均有显著差异（p﹤0.05）；颈椎病模型对照组中TNF-α水平明显偏高，与空白对照组、颈复康颗粒对照组和六白菖砂粒剂高剂量组相比均有显著差异（p﹤0.05），而与六白菖砂粒剂低剂量组（Ⅴ）相比无显著差异（p﹥0.05）。结论：六白菖砂汤能明显提高家兔血清IGF-1水平，降低TNF-α，由此来对颈椎病产生疗效。关键词：六白菖砂汤；颈椎病；骨质疏松症；IGF-1；TNF-α-1-Influenceof“LiubaichangshaDecoction”onIGF-1、TNF-αinBloodSerumofcervicalspondylosisrabbitmodelChenPeng(OrthopedicsofTraditionalChineseMedicine)DirectedbyYangHaoAbstractObjective：ToobservetheinfluenceofLiubaichangshaDecoctiononIGF-1、TNF-αinBloodSerumofcervicalspondylosisrabbitmodel,inordertoobservethecliniceffectofcuringcervicalspondylosisbythisprescriptionMethods：40adultrabbitswererandomlydividedinto5groups,controlgroup,modelcontrolgroup,Neck-recover-granulecontrolgroup,LiubaichangshaDecoctionhighdosegroupandLiubaichangshaDecoctionlowdosegroup,8rabbitseachgroup.Cervicalspondylosismodelwasmadethroughkeepingsittingandhead-bendingposition.Fur,weightandactionconditionofrabbitswereobservedintheexperiment.Alltherabbitswereexaminedwithroentgenogramanddualenergyradiographabsorptiometryforcervicalvertebraeinthe4thand12thweek,thepathologyofcervicalintervertebradiscsweremeasuredinthe12thweek,IGF-1,TNF-αinBloodSerumweredeterminedandobservedbyradioimmunoassaymethod(RIA)inthe12thweek.Results：Fur,weight,actionconditionandcervicalintervertebradiscspathologyofrabbitsinthecervicalspondylosisandLiubaichangshaDecoctionlowdosegroupweresimilar,thebonedensitydeclinedinthetwogroups;TheindexmentionedaboveofLiubaichangshaDecoctionhighdosegroupandNeck-recover-granulecontrolgroupweresimilartothoseofthecontrolgroup;IGF-1levelsweresignificantlyelevatedinLiubaichangshaDecoctionhighdosegroupthaninthecontrolgroupcervicalspondylos