丹红注射液预处理对大鼠心脏缺血再灌注损伤保护作用.docVIP

丹红注射液预处理对大鼠心脏缺血再灌注损伤保护作用 　　【摘要】 目的 探讨丹红注射液预处理对大鼠心脏缺血再灌注损伤的保护作用。方法 雄性SD大鼠24只。大鼠心脏离体后悬于Langendorff灌流装置。随机分为3组,每组8只：缺血组(ISCH组)：大鼠心脏离体平衡50 min，37℃缺血45 min，K?H液复灌3 h；低浓度丹红注射液预处理组(L?prec组)：大鼠心脏平衡20 min，以含低浓度丹红注射液(5 mg/L)的灌注液灌注15 min，洗脱15 min，37℃缺血45 min，K?H液复灌3 h；高浓度丹红注射液预处理组(H?prec组)：大鼠心脏平衡20 min，以含高浓度丹红注射液(15 mg/L)的灌注液灌注15 min，洗脱15 min，37℃缺血45 min，K?H液复灌3 h。平衡50 min和复灌2 h 期间连续监测LVDP，LVDEP，±dp/dtmax。复灌60 min时接取冠脉流出液，测定冠脉流出液中CK和LDH的含量。复灌结束，将心脏切片(1～2 mm)并做TTC染色。结果 机械功能：基础状态时3组LVDP，LVDEP，±dp/dtmax两两比较差异无统计学意义(P0.05)。复灌期间L?prec组的LVDP，LVDEP，±dp/dtmax与ISCH组相比，差异无统计学意义。H?prec组的LVDP和±dp/dtmax与ISCH组相比，显著升高(P0.05)。结论 丹红注射液预处理能减轻心肌缺血再灌注损伤后CK和LDH 的释放，缩小心梗面积；高浓度丹红注射液预处理能显著增强心脏缺血再灌注损伤后心脏的机械功能。? 　　【关键词】丹红注射液；预处理；缺血再灌注损伤 　　?? 　　Danhong Injection protects against myocardial reperfusion injury in isolated rat heart WANG Cong?zhen,CAI Shu?fang.Third Clinical Hospital of Weifang Medical Unirersity,Weifang 262500,China 　　【Abstract】 Objective 　　This study investigated whether Danhong Injection could protect perfused hearts of rats against ischemia/reperfusion injury (IR) when it was administered before the adverse ischemic event. Methods Studies were conducted in buffer?perfused isolated rat hearts. This experiment consisted of baseline perfusion for 20 min, warm global ischemia for 40 min and 2 hours of reperfusion.Preconditioning of hearts in L?prec and H?prec group was induced by 15 min of low(5 mg/L) or high(15 mg/L) concentration of Danhong Injection followed by 15 min of wash out. Untreated hearts served as ischemic control (ISCH) group. Left ventricular mechanical function (LVDEP, LVDP, ±dp/dtmax), cardiac enzyme activity(CK, LDH), and infarct size (IS) were measured as variables of ventricular function and cellular injury, respectively. Results Danhong Injection preconditioning attenuated the release of CK and LDH and reduced the IS of the heart during reperfusion, compared with ISCH group(P［1,2］。但应用丹红注射液进行预处理对于心肌缺血再灌注损伤的保护作用国内外尚未见报道。因此, 我们利用大鼠离体灌流模型，研究丹红注射液预处理对心脏发生37℃缺血40 min后