分子遗传学反转录转座子.docVIP

分子遗传学-反转录转座子 volve alternation of reverse transcription from RNA to DNA with transcription from DNA to RNA. Only retroviruses can generate infectious particles. Retroposons are confined to an intracellular cycle. ?The retrovirus life cycle involves transposition-like events ? reverse transcriptase integrase RNA→DNA →RNA The genes of the retrovirus are expressed as polyproteins that are processed into individual products. 5% 反转录病毒颗粒示意图 (P295) gag gag gag env env pol pol pol ◆ 病毒RNA的每一个末端都有短重复序列(R), 因而5`和3`端分别称为R-U5和U3-R； ◆ tRNA引物结合到5`端的100~200 bp位点后，反转录酶开始合成负链DNA； ◆ 当酶到达末端，RNA的5`端就降解，接着露出DNA产