干旱草原豆科植物根瘤菌物种多样性分析及应用试验-species diversity analysis and application test of rhizobia isolated from leguminous plants in arid grassland.docxVIP

Abstract nodules of Caragana korshinskii)， 3136771 (isolated from the nodules of Astragalus sp in ZhangJiaKou)， 3136921 (isolated from the nodules of Phaseolus vulgaris in ZhangJiaKou)， which belonged to Sinorhizobium; The strain 3137033 (isolated from the noldus of Vicia Sepium in ZhangJiaKou)， 3136961 (isolated from the noldus of Melissitns sp.in ZhangJiaKou) belonged to Rhizobium; The strain 3137021 (isolated from the nodules of Melilotus suaveolens in Shanxi)， 3526632 (isolated from the noldus of Astragalus sp. in Shanxi) belonged to Ochrobactrum; The strain 3526732 (isolated from the noldus of Lespedeza bicolor in Shanxi) belonged to Phyllobacterium. The physiological and chemical characteristics of all isolates were tested and chose two strong resistance Rhizobial strains (SX01 and HB02) were used to carry out the inoculation experiment. The inoculation effect and the total tumor rate were tested and calculated by the way of BOX-PCR molecular markers. The field inoculation experiments in Shanxi show that the inoculated Rhizobia SX01 and HB02 significantly improved the plant growth (P0.05)， such as the plant height， branches， leaf area， dry weight and the number of effective nodules. The results showed that inoculated Rhizobia (SX01 and HB02) plantsgrowing characters (the plant height， branches， leaf area， dry weight and the number of effective nodules) were increased by 11.7%， 12.5%， 30.0%， 34.9%， 21.5% and 10.1%， 10.2%， 28.3%， 34.2%， 19.6%， respectively. In order to test the inoculation effect of rhizobial strains and screen high efficient rhizobia strains， we studied the competitive nodulation ability of SX01 and HB02 by BOX-PCR Molecular Marker Method. The rhizobia strains reisolated from root nodules of alfalfa and inoculation stains were analyzed by BOX-PCR， the nodule occupancy of inoculation stains were detected by comparing BOX molecular fingerprint. The results showed that the nodule occupancy of the two strains (SX01