新血府逐瘀汤对动脉粥样硬化大鼠主动脉斑块的形成及th fth失衡影响.docxVIP

Effect of Renewed Xuefuzhuyu Decoction on the formation of Aorticplaque and the imbalance of Th1/Th2 Cytokine in AtherosclerosisRatsSpeciality:Traditional chinese medicineAuthor: Wang YongTutor: Ji ZhongqiangAbstractObjective：To observe the effect of Renewed Xuefuzhuyu Decoction on theformation of Aortic plaque and the imbalance of Th1/Th2 Cytokine in AS rats ,andresearch the mechanism of Renewed Xuefuzhuyu Decoction preventing AS.Methods： 65 SPF Wister male rats were randomly divided into five groups: Theblank group, model group, Chinese medicine in low dose group and high dose group,western medicine group. Except the blank group, other groups were made into AS modelby feeding high-fat diet and injecting vitamin D3 into abdominal cavity. The drug wasgiven through irrigation stomach in each group. After 8 weeks ,IFN-r、IL-4 in serum wasmeasured by using ELISA, then the ratio of IFN-r / IL-4 will be calculated. The intima andmedia thickness of Thoracic aortic was measured through Microscope, and then observethe change of Thoracic aortic pathology through microscope.Result：Xuefuzhuyu Decoction can decrease the thickness of the intima and mediaof Thoracic aortic ,can improve the Aortic pathology. Campared with the model group,thecontent of IFN-r in serum was reduced, and IFN-r in serum was increased. the function ofTh1 / Th2 cell offset to the Th2 .Conclusion：Renewed XuefuZhuyu Decoction can inhibit the immuneinflammatory reaction in AS, prevent the formation of AS though regulating the functionimbalance of Th1/Th2 cell.cellsKey Wards：Renewed Xuefuzhuyu Decoction; Atherosclerosis; Th1 cells; Th2目录引言 ............................................................................................................................... 1动物实验 ............................................................................................................................... 3一、实验材料 ...........................................................................................................