新 疆人类8型疱疹病毒的致病机制分析及在献血者中流行病学调查-analysis of pathogenic mechanism of human herpesvirus 8 in xinjiang and epidemiological investigation among blood donors.docxVIP

was significantly lower than other types of KS (p, 0.025), in concordance with distribution of anti-HHV-8 antibodies. The common up/down regulated genes between two samples were 117, mainly related with HIV, hyperplasia, skin cancer, inflamation and melanoma those known factors attributed to HHV-8 infection or KS pathogenesis. Conclusions : HHV-8 mixed-antigens ELISA was strong technique platform for large scale epidemiology research. The seroprevalence of HHV-8 in blood donors from Xinjiang was great higher than same population in other provinces. Thus to detect anti-HHV-8 antibodies before donation became necessary. Ethnic minority background was the most crucial risk factor resulting in HHV-8 infection and high titre antibodies. The genotype of local HHV-8 and its distribution closely related with ethnic origin and evolution of local minorities. Different genotype of HHV-8 could have different pathogenesis mechanism, thus provided foundation for subsequently genetic susceptibility research. Highly sensitive and specific real-time fluorescent PCR for HHV-8 detection was contructed successfully, being good candidate for suspicious cases confirmation. HHV-8 viral load in local AIDS-KS cannot be used to predict the tumor progression. The inportance of the index should be evaluated differently among AIDS-KS/Classic KS/transplantion-associated KS. Gene expression profiling showed endoplasmic reticulum stress signal pathway make critical role during KS pathogenesis.[Key words] Human Herpesvirus 8 (HHV-8);Kaposi’s Sarcoma (KS);Blood Donors; Risk Factor;Genotype;Viral Load;Expression Profile前言人类 8 型疱疹病毒（human herpesvirus 8，HHV-8），又称卡波西氏肉瘤相关疱疹 病毒（Kaposi’s sarcoma-associated Herpesvirus，KSHV），是疱疹病毒家族的最新成员。 已证实它是高度致癌的 DNA 病毒，与多种 艾滋（ Acquired Immunodeficiency Syndrome，AIDS）相关肿瘤包括卡波西氏肉瘤（Kaposi’s sarcoma，KS）、原发弥漫 性淋巴瘤（Primary Effusion Lymphoma，PEL）、多中心卡斯特莱曼病（Multicenter Castleman’s Disease，MCD）等的发生密切相关[1]。其中两类与免疫缺陷或抑制密切 相关的 KS 类型（AIDS 和器官移植 KS），具备发病迅速、病程严重且常累及各脏器 因此致死率和致残率很