n乙酰半胱氨酸对大鼠急性脊髓损伤后nfκbp65及前炎性因子表达影响-effect of n - acetylcysteine on expression of nf κ bp65 and proinflammatory factor after acute spinal cord injury in rat.docx

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n乙酰半胱氨酸对大鼠急性脊髓损伤后nfκbp65及前炎性因子表达影响-effect of n - acetylcysteine on expression of nf κ bp65 and proinflammatory factor after acute spinal cord injury in rat.docx

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n乙酰半胱氨酸对大鼠急性脊髓损伤后nfκbp65及前炎性因子表达影响-effect of n - acetylcysteine on expression of nf κ bp65 and proinflammatory factor after acute spinal cord injury in rat

ofchangesoccur,suchasneuralcellsapoptosis,glialcellsproliferation,formationofglialscarandcavityatinjuredsite.ThespinalcordofthegroupAhadnosignofinflammatory.3.Immunohistochemistryresults：TheexpressionofNF-κBp65haslittlechangeingroupAatsixobservationpoints.TheexpressionofNF-κBp65proteinweremarkedlyincreasedingroupBandCat6hafterspinalcordinjury,andreachedthepeakat12hafterinjury,thenbeganaslowdeclineat1d,andstillkeptahighlevelat3dafterinjury,thenreturnedtonormalat7dafterinjury.TheexpressionofNF-κBp65ingroupBwassignificantlyhigherthanthatofgroupCat6h,12h,1d,and3dafterSCI,whichhadstatisticallysignificance(p0.05).TheexpressionchangeofNF-κBp65ingroupBandChavesametendency.Similarly,fewTNF-α,IL-1βandIL-6positiveexpressionwerefoundingroupA.TheexpressionsofTNF-α,IL-1βandIL-6ingroupBandCweresignificantlyincreasedatearlystageofacuteinjuryspinalcord,andreachedthepeakat12h-1dafterinjury,thendecreasedslowlyat3dafterinjury.TheexpressionofTNF-α、IL-1βandIL-6werehigheringroupBthaningroupCat6h,12h,1d,and3dafterSCI,whichhadstatisticallysignificance(p0.05).4.Westernblotdetection:ThegroupAshowedasmallamountofNF-κBp65proteinexpression.TheexpressiontendencyofNF-κBp65ingroupBandCwereconsistentinbothWesternblotandimmunohistochemistrydetection.TheexpressionofNF-κBp65proteinwereincreasedat6hafterspinalcordinjury,andreachedthepeakat12h-1d,thendecreasedslowlyandreturnedtonormalat7dafterinjury.TheexpressionofNF-κBp65ingroupBwasmuchhigherthaningroupCat6h,12h,1d,and3dafterspinalcordinjurywithstatisticallysignificantdifference(P0.05).Conclusion:N-acetylcysteinecansignificantlysuppresstheactivationofNF-κBp65anddown-regulatetheexpressionsofpro-inflammatoryfactorssuchasTNF-α、IL-1βandIL-6inratsafterspinalcordinjury,andreducethesecondaryinflammatoryreactionsafterspinalcordinjury,andexertneuroprotectiveeffectsondamagedspinalcord.Keywords:rat;spinalcordinjury;N-acetylcysteine;NF-κB;TNF-α;IL-1β;IL-6英文缩写词表英文缩写英文全称中文全称ASCIAcutespinalcordinjury急性脊髓损伤PSCIPrimaryspinalcordinjury原发性脊髓损伤SSCISequentialspinalcordinjur