rack1与nfκb信号通路相关蛋白在幽门螺杆菌感染相关胃黏膜病变中的表达-expression of rack 1 and nf κ b signaling pathway related proteins in gastric mucosal lesions associated with helicobacter pylori infection.docxVIP

摘要κBα蛋白表达高于慢性非萎缩性胃炎组，有显著差异（40%vs77.5%，p0.01）。（3）与Hp感染的关系：将慢性非萎缩性胃炎组、癌前病变组各自分为Hp阳性和阴性组，进行组间比较。结果显示在胃黏膜病变早期阶段，Hp阳性组RACK1显著高于Hp阴性组，差异有统计学意义（100%vs90%；87.5vs90%，p0.05），而Hp阳性和阴性组间IκBα与p-IκBα蛋白表达无显著性差异（p0.05）。（4）胃癌组织中RACK1与IκBα、p-IκBα表达的关系：胃癌组织中RACK1与IκBα蛋白表达正相关（p0.01），与p-IκBα负相关，差异均有统计学差异（p0.01）。2.Hp作用GES-1后RACK1与NF-κB信号通路相关蛋白表达情况Hp活菌（MOI=50：1）作用GES-1细胞后，RACK1表达量在作用后30min开始升高，3h达到高峰（p0.01），6h开始下降（p0.05）。NF-κB信号通路相关蛋白中p65表达无明显变化（p0.05），而p-p65(Ser276)蛋白表达水平在各时间点均明显升高，30min开始升高，3h达到高峰（p0.01），6h内均高于0h（p0.05），p-p65(Ser536)表达水平在3h显著升高（p0.01），作用6h时仍高于0h（p0.05），结论：1.RACK1表达下调和NF-κB信号通路活化可能参与了胃黏膜癌变过程。2.Hp感染相关胃粘膜癌变早期RACK1表达上调，这是否参与了Hp相关胃黏膜癌变发生尚待进一步探讨。关键词：胃粘膜癌变；幽门螺杆菌；RACK1；NF-κBABSTRACTBackgroundandAim:Gastriccanceristhefifthmostcommonmalignanttumorintheworld,leadingtothethirdtumorassociateddeath，nearlyonemillionnewcasesincreasingperyear.Seventypercentofthefinancialburdenfallsinthedevelopingcountries,especiallyinAsia.Hpinfectionisthemainriskfactorofgastriccancer.AlargenumberofresearcheshavedemonstratedthatHpplaysimportantrolesintheinitiationandtheprogressionofthegastricmucosalcancerationtheprocessofwhichincludethechronicatrophicgastritis,intestinalmetaplasia，dysplasiaandgastriccancer.However,theunderlyingmechanismofHpinfectioningastriccancerstillremainsunclear.MostrelatedstudiesrevealedthattheNF-κBsignalpathwayisactivatedaftertheinfectionofHp,whichsubsequentlyiscloselyrelatedwiththegastritis,gasriculcerandtheincidence,development,survivalandprognosisofgastriccancer.NF-κBisanimportantmultifunctionalnucleartranscriptionfactor.Intherestingstate，theNF-κBdimerp50/p65combineswiththeinhibitoryproteinsIκBtoformacomplexandexsitinthecytoplasminaninactivatedstate.ManyextracellularstimulileadtothephosphorylationofIκBproteinsandpromotetheirubiquitinationandsubsequentdegradationbyproteasome.Thisprocessleadstothetranslocationofp65fromthecytoplasmtothenucleus,whereitinitiatesthetranscriptionofaseriesoftargetgenes.Thenitplaysanimportantroleintheinflammation、immunityandtumor.Therefore,itmayprovidene