rgc 32在胰腺癌 emt 中的作用及机制研究-role and mechanism of rgc 32 in emt of pancreatic cancer.docxVIP

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rgc 32在胰腺癌 emt 中的作用及机制研究-role and mechanism of rgc 32 in emt of pancreatic cancer.docx

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rgc 32在胰腺癌 emt 中的作用及机制研究-role and mechanism of rgc 32 in emt of pancreatic cancer

独创性声明本人郑重声明，本学位论文是本人在导师指导下进行的研究工作及取得的研究成果的总结。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人和集体，均已在文中以明确方式标明。本人完全意识到本人将承担本声明引起的一切法律后果。学位论文作者签名：日期：年月日学位论文版权使用授权书本学位论文作者完全了解学校有关保留、使用学位论文的规定，即：学校有权保留并向国家有关部门或机构送交论文的复印件和电子版，允许论文被查阅和借阅。本人授权华中科技大学可以将本学位论文的全部或部分内容编入有关数据库进行检索，可以采用影印、缩印或扫描等复制手段保存和汇编本学位论文。本论文属于保密□ ，在年解密后适用本授权书。不保密□。（请在以上方框内打“√” ）学位论文作者签名：指导教师签名：日期：年月日日期：年月目录英文缩略词表 ·······························································································1中文摘要 ·······································································································4英文摘要 ·······································································································6 正文 ·······································································································9 前言 ·······································································································9材料和方法 ·························································································12结果 ·····································································································35讨论 ·····································································································56参考文献 ·····························································································60 综述 ·····································································································65 附录 1：攻读期间发表论文情况 ·····················································87 附录 2：攻读期间获奖情况 ·····························································88 致谢 ·····································································································89英文缩略词表英文缩写英文全称中文名称EMTepithelial-mesenchymal transition上皮间质转化TGF-βtransforming growth factor-β转化生长因子-βRGC-32response gene to complement-32补体反应基因-32SPstreptavidin-perosidase链霉亲和素-过氧化物酶H2O2hydrogen peroxide双氧水PBSphosphate-buffered saline磷酸盐缓冲溶液DABdiaminobenzidine二氨基联苯胺ddH2Owater distillated twice双