BRMS1HDAC1VEGF-C在乳腺癌组织中表达及意义.docVIP

BRMS1HDAC1VEGF-C在乳腺癌组织中表达及意义 　　[摘要] 目的 探讨BRMS1、HDAC1及VEGF-C 蛋白在乳腺癌、乳房纤维瘤及正常乳腺组织的表达及意义。方法 采用免疫组织化学方法随机检测60例乳腺癌组织标本BRMS1、HDAC1、VEGF-C蛋白的表达,随机收集乳房纤维瘤组织及其旁正常乳腺组织各20例作为对照组,结合临床病理资料分析其临床意义。结果 BRMS1 在乳腺癌组织中表达率为60%,在乳房纤维瘤组织中表达率为85%,在正常乳腺组织中表达率为95%,乳腺癌组织中的BRMS1的表达明显低于乳房纤维瘤和正常乳腺组织(P 　　[关键词] BRMS1; HDAC1; VEGF-C; 乳腺癌; 免疫组织化学 　　[中图分类号] R737.9 [文献标识码] A [文章编号] 1673-9701(2010)09-10-03 　　 　　Expression and Significance of BRMS1,HDAC1 and VEGF-C in Breast Cancer 　　XIA Genyu CHEN Bin 　　Yijishan Hospital of Wannan Medical College,Wuhu 241001,China 　　 　　[Abstract] ObjectiveTo investigate the expression and clinical significance of breast cancer metastasis suppressor 1(BRMS1)protein,histone deacetylase 1(HDAC1) and vascular endothelial cell growth factor-C(VEGF-C) protein in breast cancer. MethodsThe expression of BRMS1 protein,HDAC1 and VEGF-C was detected by using immunohistochemical technique in 60 cases of breast cancer,20 cases of breast fibroids and 20 cases of adjacent normal breast tissue as control and the clinical significance was analyzed with histopalthological records. ResultsThe positive rate of BRMS1 expression was 60% in breast cancer tissue,85% in breast fibroids and 95% in normal breast tissue,showing the positive rate of BRMS1 in breast cancer was much lower than that in breast fibroids and normal breast tissue(P2cm 39例;肿瘤直径≤2cm 21例,无淋巴结转移者20例;有淋巴结转移者40例;根据TNM分期,I期9例,II期27例,III期21例,IV3例ER阳性32例,ER阴性28例,PR(+)44例,PR(-)16例,Her-2(-)～(+)41例,Her-2(2+)～(3+)19例,随机收集良性乳房组织经病理证实均为乳房纤维腺瘤20例,随机收集乳房纤维腺瘤旁0.5cm外的正常乳腺组织20例作为对照。 　　1.2 标本采集和石蜡切片制备 　　乳腺癌和乳房纤维腺瘤蜡块均来自病理科,收集我院门诊乳房纤维瘤旁正常乳腺组织(距纤维瘤0.5cm以上),以10%甲醛固定,石蜡包埋后制成蜡块,全部蜡块以4μm连续切片,行常规HE染色,选取形态典型标本行免疫组化染色。 　　1.3 主要实验试剂 　　BRMS1兔抗人单克隆抗体,工作浓度1:100。HDAC1鼠抗人多克隆抗体,即用型。即用型SABC免疫组化染色试剂盒,均购自武汉博士德公司。VEGF-C兔抗人单克隆抗体和DAB显色试剂盒,购自北京中杉金桥生物技术有限公司。 　　1.4 制片检测 　　正常乳腺组织收集后经福尔马林液固定,24h内取材,常规脱水、透明、浸蜡、包埋,切片(厚度为4μm),制作成石蜡切片。免疫组化(SABC法)严格按试剂盒说明操作。设阳性对照和阴性对照,以已知的乳腺癌阳性片为阳性对照,以PBS代替一抗的乳腺癌组织免疫组化染色结果为阴性对照。 　　阳性判定:染色主要位于细胞胞浆或(和)胞核内,阳性表达