不同时期骨髓间充质干细胞移植对大鼠肝纤维化作用.docVIP

不同时期骨髓间充质干细胞移植对大鼠肝纤维化作用 　　[摘要] 目的 探讨骨髓间充质干细胞移植对大鼠肝纤维化的作用,并探索最佳移植时期。方法 应用贴壁培养法分离SD大鼠MSCs,分别于肝硬化造模第6周,10周经尾静脉注射1×106MSCs。在移植4周后,评价移植组与对照组的生存状态,行肝功能检测(ALB,ALT,TBIL),肝脏HE染色。结果 MSCs经尾静脉移植入肝硬化大鼠后,大鼠肝功能均明显改善,与对照组相比有统计学意义(P 　　[关键词] 骨髓间充质干细胞; 移植; 肝纤维化; 大鼠 　　[中图分类号] R329.2 [文献标识码] A [文章编号] 1673-9701(2010)05-11-02 　　 　　Different Periods of Bone Marrow Mesenchymal Stem Cells Transplantation:Effects on Rat Liver Fibrosis 　　ZHANG Xiaoliang1 SHANG Zhonghua2 WANG Mancang1 JIA Chenle1 　　1.Shanxi University,Taiyuan 030001,China;2.Department of General Surgery,the Second Hospital of Shanxi Medical University,Taiyuan 030001,China 　　 　　[Abstract] ObjectiveTo investigate the effects of transplanted mesenchymal stem cells(MSCs) on the development of hepatic fibrogenesis in rats,and explore the best transplanting period. MethodsSD rat mesenchymal stem cells(MSCs) were isolated by adherent culture,and the tail vein injection of 1×106MSCs was given at the first 6 weeks and 10 weeks of Cirrhosis modeling. At 4 week after MSC transplantation,the survival state was evaluated,the liver functions(ALB.ALT.TBIL.)were detected,and the liver HE staining was carried out in the transplant group and control group. ResultsThe rat liver function improved noticeably after MSC transplantation,with a significant difference between the transplant group and control group. Conclusion MSCs transplantation can improve the biochemical characteristics of rat peripheral blood and liver histological structure,and the transplantation at the early stage of liver fibrosis may have a better result. 　　[Key words]Bone marrow mesenchymal stem cells; Transplantation:Liver cirrhosis; Rat 　　 　　肝纤维化是多数终末期肝病发展的共同阶段,这一阶段若得不到有效治疗,最终会发展为肝硬化,甚至导致患者死亡。对于终末期肝病,原位肝移植(orthotopic livert ransplantation,OLT)理论上应是最为理想的治疗选择,但是供体的紧缺以及OLT后免疫排斥反应与高额的治疗费用等又成了一个新的社会问题,即使更新的异位肝移植和原位部分肝移植也存在同样的临床问题[1],因此必须寻找其他可能的解决办法。而干细胞在一定条件下可以分化为肝样细胞,且不存在免疫排斥,为临床终末期肝病治疗提供了新的思路。 　　1 材料与方法 　　1.1 材料 　　1.1.1 实验动物及分组 普通级SD大鼠,5～6周龄,体重80g左右,用于MSCs的制备。成年洁