丙型肝炎病毒基因分型与肝病程度关系研究.docVIP

丙型肝炎病毒基因分型与肝病程度关系研究 　　【中图分类号】R373.2 【文献标识码】A【文章编号】1005-2720(2010)10 - 07 - 02 　　【摘要】目的 探索丙型肝炎病毒(HCV)基因型在辽宁省大连地区的分布及流行的优势型,并探讨HCV基因型与肝病程度的关系。方法 采用型特异性引物逆转录巢式PCR法和限制性片段长度多态性分析(RFLP)法对85例抗-HCV阳性的慢性丙型肝炎、丙肝肝硬化、丙肝相关肝癌病人进行 HCV的基因分型。结果 85份抗HCV阳性标本中,HCV RNA检出率为61例,占71.76%(61/85)。在85份抗HCV阳性血清中,仅检出了61例1b和2a型感染者,其中1b型32例,占52%(32/61);2a型29例,占48%(29/61)。结论 大连地区基因型为1b型和2a型两种型,符合中国丙肝病毒基因分型,大连地区1b型和2a型基本相等。未发现1b和2a基因型因肝病严重程度而有所不同。 　　【关键词】肝炎病毒;丙型;基因分型;型特异性引物 　　Study on Hepatitis C Virus Genotype in Dalian 　　Xie YingXie Chen 　　(Department of Infectious Disease of The Second Affiliated Hospital of Dalian Medical University, 　　Dalian liaoning116027) 　　【Abstract】Objective In order to find out the distribution and prevalence of HCV genotype in Dalian, Liaoning province, and make a research on the connection of HCV genotype and the degree of liver disease. Methods To process with HCV gene classification to the 85 cases which have anti-HCV positive chronic hepatitis C, hepatitis C cirrhosis, hepatitis C cancer respectively by means of RT-nested-PCR with primere deduced from 5 ′non-coding regionand restriction fragment length polymorphism. Results In the 85 anti-HCV positive cases, detection ratio of HCV RNA are 61cases, which account for 71.76% (61/85). In those 85 anti-HCV positive blood serum, 61cases are detected with 1b and 2a, 32 cases with 1b which account for 52% (32/61), 29 cases with 2a which account for 48% (29/61). Conclusion There are two HCV genotypes in Dalian, namely, 1b and 2a, which matches with HCV genotype in China. In Dalian, 1b and 2a are equivalent to each other. It is not discovered that there are any differences between 1b and 2a because of the severity degree of hepatitis. 　　【Key words】Hepatitis C virus;genotyping;Type specific primer 　　 　　研究旨在了解丙型肝炎病毒(HCV)基因型在辽宁省大连地区的分布及流行的优势型,并探讨HCV基因型与肝病程度的关系。 　　1 对象与方法 　　1.1 对象选取2009年1月初至2009年12月底期间于大连医科大学附属二院门诊病例以及住院病例,抗-HCV阳性的慢性丙型肝炎、丙肝肝硬化、丙肝相关肝癌病人共85例。病变程度的判断依据2000年中华医学会传染病与寄生虫学分会、肝病学分会联合修订的《病毒性肝炎防治方案》中关于肝脏炎症和纤维化