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A fast stretching coordinate, corresponding to reduced bond order Rotation about the phenolic single bond Temperature dependent The distribution about the phenyl single bond Massimo Olivucci et. al J. AM. CHEM. SOC. 2004, 126, 5452 Ab initio CASPT2//CASSCF relaxation path computations Fernando Bernardi et. al J. AM. CHEM. SOC. 2005, 127, 3952 Conical intersection dynamics in solution Population on S1 as a function of time T. J. Martinez et. al Faraday Discuss, 2004, 127, 149 Full multiple spawning (FMS) nonadiabatic wavepacket dynamics method CASSCF/CASPT2/AM1 ① 4, Some new events Jye-Shane Yang et. al Chem. Commun., 2008, 1344 The phenyl derivative Jye-Shane Yang et. al Chem. Commun., 2008, 1344 Jye-Shane Yang et. al Chem. Commun., 2008, 1344 ② Liangxing Wu et. al J. AM. CHEM. SOC. 2008, 130, 4089 Lock ? Liangxing Wu et. al J. AM. CHEM. SOC. 2008, 130, 4089-4096 Compare quantum efficiency Liangxing Wu et. al J. AM. CHEM. SOC. 2008, 130, 4089-4096 ③ Jian Dong et. al J. AM. CHEM. SOC. 2009, 131, 662 Crystal phase p-HBDI Jian Dong et. al J. AM. CHEM. SOC. 2009, 131, 662 ④ Thank you for your attention! Deactivation Mechanism of the Green Fluorescent Chromophore 2010/4/2 Bioluminescence Osamu Shimomura Prasher’s two good idea Expression of the fluorescent GFP FP Family The palette of mutated FPs The Nobel Prize Morise, H.; Shimomura, O.; et.al Biochemistry 1974, 13, 2656 Absorption and fluorescence spectrum of GFP Fan Yang, Larry G. Moss et.al Nature biotechnology 1996, 14, 1246 Crystal structure of GFP K.Brejc et.al PNAS USA 1997, 94, 2306 Satoshi Kojima, et.al. Tetrahedron Letters 1998, 39, 5239 p-HBDI showed weak fluorescence Haruki niwa et,al Proc. Natl. Acad. Sci. USA 1996 ,93, 13617 Temperature dependence of the fluorescence spectrum of p-HBDI An important question How does the protein convert such a poor fluorophore to the brightly fluorescent GFP? A clear answer to this question could assist in designing brighter GFP variants 2, Experiment research F
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