有氧运动结合茶多酚对高脂膳食大鼠肝脏抗氧化能力影响.docVIP

有氧运动结合茶多酚对高脂膳食大鼠肝脏抗氧化能力影响 　　摘 要： 目的：观察有氧耐力运动及茶多酚补充对高脂膳食大鼠肝脏抗氧化能力的影响。方法 ：雄性SD大鼠48只，随机分为6组：安静对照组、高脂膳食组、运动对照组、高脂膳食运动 组、高脂膳食低茶多酚运动组、高脂膳食高茶多酚运动组。检测大鼠肝脏T-AOC、SOD、CAT 、NOS活性及MDA、NO含量。结果：高脂膳食大鼠肝脏SOD、CAT活性及MDA含量显著高于对照 组；高脂膳食运动组SOD、CAT活性较高脂膳食组显著上升，MDA含量显著降低；高脂膳食茶 多酚运动组SOD活性升高、CAT活性下降，MDA恢复至安静对照组水平。高脂膳食大鼠肝脏TNO S、iNOS活性及NO含量都比安静对照组显著增加；高脂膳食运动组三者较高脂膳食组显著下 降。与安静对照组比较，其它5组T-AOC均显著提高，其中茶多酚补充与运动联合作用提高幅 度最大。结论：有氧耐力运动可以提高高脂膳食大鼠肝脏抗氧化能力，有氧耐力运动联合茶 多酚补充对高脂膳食大鼠肝脏抗氧化能力的提高效果优于单纯的有氧耐力运动。 　　关键词：有氧耐力运动；茶多酚；高脂膳食；肝脏；抗氧化能力 　　中图分类号：G804.7 文献标识码：A 文章编 号：1007-3612(2011)11-0062-04 　　Effects of Aerobic Endurance Training and Tea Polyphenols on the Anti?oxidative Capability of the 　　Liver Cells of Fat?rich Diet Rats 　　LUO Yan?rui?1，WANG Jian?she?2，LI Xin?3，JI Liu?1 　　（1.Sport Health College，East China Normal Universit y，Shanghai 200241，China; 2.P.E.Dept.， 　　Henan Normal University，Xin xiang 453007，Henan China; 3.Beijing Sport University，Beijing 100084，China） 　　Abstract: Objective: To observe the influence of aerobic endurance exercise and tea polyph enols on the liver of fat?rich diet rat’s metabolize of reactive oxygen specie s (ROS) and reactive nitrogen species (RNS).Methods: 48 healthy male SD rats we re randomly divided into six groups: Group A (quietness control group)，Group B (fat?rich diet group)，Group C (exercise control group)，Group D (exercise+fa t?rich diet group)，Group E (exercise + fat?rich diet + low tea polyphenols gr oup)，Group F (exercise + fat?rich diet and high tea polyphenols group).The au thors detected the activity of total anti?oxidative capacity (T?AOC)，super ox ide dimutese (SOD)，catalase (CAT)，total nitric oxide synthase (TNOS)，inducibl e nitric oxide synthase (iNOS) and the content of malonaldehyde (MDA)，nitric ox ide (NO) in the rat’s liver?cell.Results: The activity of SOD and CAT，and th e content of MDA in the fat?rich diet group rat’s liver?cell were significant ly higher than that in the quietness control group.The activity of SOD and CAT in exercise + fat?rich