吉西他滨对人乳腺癌mcf-7细胞株cn-ⅱ aperef-1 mrna和蛋白表达的影响-effect of gemcitabine on mrna and protein expression of human breast cancer mcf - 7 cell line cn - ⅱ aperef - 1.docxVIP

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吉西他滨对人乳腺癌mcf-7细胞株cn-ⅱ aperef-1 mrna和蛋白表达的影响-effect of gemcitabine on mrna and protein expression of human breast cancer mcf - 7 cell line cn - ⅱ aperef - 1.docx

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吉西他滨对人乳腺癌mcf-7细胞株cn-ⅱ aperef-1 mrna和蛋白表达的影响-effect of gemcitabine on mrna and protein expression of human breast cancer mcf - 7 cell line cn - ⅱ aperef - 1

Ⅱ、APE/Ref-1 表达水平从第十天起明显增加，第十六天达到最高。并且干预至第 10、13、16 天后存活的相对耐药细胞中的 APE1 和 CN-ⅡmRNA 和蛋白表达水平与非耐药细胞相比，结果具有统计学意义。（P＜0.05）结论：CN-Ⅱ，APE/Ref-1在乳腺癌化疗时表达明显增强，可能与化疗耐药性的产生有关，并提示针对CN-Ⅱ，APE/Ref-1的靶向干预可能有助于提高乳腺癌的化疗敏感性。关键词：吉西他滨；CN-Ⅱ；APE/Ref-1；乳腺癌；化疗耐药性Effects of Gemcitabine on Expression of CN-Ⅱ, APE/Ref-1mRNA and Protein in Human Breast Cancer Cell Line MCF-7ABSTRACTThe incidence of breast cancer is the highest in female malignant tumor diseases incidences. And there is a rising trend. After operation, chemotherapy and other comprehensive treatment, early breast cancer gets good therapeutic results, while advanced breast cancer loses the chance of operation and has to receive chemotherapy. At present, chemotherapy with anthracycline and taxane is the best choice for advanced breast cancer. Although the effect is significant, there is still 20%~30% patients who have drug resistance. In the treatments of advanced breast cancer, the follow-up treatments for drug resistant patients is a difficult problem for the current clinical science. As the third generation chemotherapy drugs such as gemcitabine, capecitabine and vinorelbine appear, the effect of chemotherapy for advanced breast cancer has increased. How to improve the efficienty of chemotherapy has become one of the most urgent problems. The present study is focusing on the selection of the most appropriate treatment according to the different individual characteristics of patients, called the individual treatment. The individual treatment not only can improve the efficacy of chemotherapy in a certain extent, but also avoid using invalid drug which would bring physical and economic loss of patients. In recent years, with the development of molecular pharmacology and molecular biology, people have a further understanding of the mechanism of tumor drug resistance, the different individual response to drug treatment and prognosis which is leaded by abnormal gene expression. Thus people realize it soberly that is the basis and condi