选择性COX抑制剂对放射所致DNA损伤修复的作用的论文.docVIP

选择性COX2?抑制剂对放射所致?DNA损伤修复的?作用的论文 选择?性COX2抑制?剂对放射所致DN?A损伤修复的作用?的论文 【摘?要】 ?目的 选择性co?x2抑制剂ce?lecoxib对?放射引起的dna?损伤修复的影响。?方法 流式细胞仪?subg1法检?测凋亡率和细胞周?期；单细胞凝胶电?泳技术检测dna?的损伤及修复。r?tpcr和we?stern bl?ot检测dna修?复基因ku70、?ku80的表达。?结果 celec?oxib对 he?la细胞的周期分?布无明显改变，但?可引起剂量依赖性?凋亡。celec?oxib可明显提?高放射引起的凋亡?率，剂量增强比（?def）在60、?80、100μm?ol/l时分别为? 3. ?1 8、 ? 4. 1 ? 6、 6?.96。同时ce?lecoxib对?6gy x线引起?的g2/m期阻滞?有明显的减弱作用?（放射组：g2/?m 32.35%?，药放组：g2/?m 5.9?5%）；cele?coxib 不明?显增加放射引起的?dna损伤，但可?明显延缓放射引起?的ssb和dsb?的修复（p 0.?05）；cele?coxib明显抑?制hela细胞k?u70、ku80?的表达（p 0.?05）。结论 c?ox2 选择性?抑制剂celec?oxib对肿瘤细?胞的放射增敏机制?可能与抑制dna?损伤修复有关，其?作用可能通过减弱?辐射诱导的g2/?m期阻滞和抑制d?sb重组修复酶d?napk成分k?u的表达得以实现?。 ? 【关键词?】 ? ce?lecoxib；?hela细胞；放?射增敏机制；细胞?周期；dna损伤?修复 effec?ts of se?lective ?cox2 in?hibitor ?on dna r?epairing? after i?rradiati?on xu hu?iting, ?yu shiy?ing, xia? shu,xio?ng hua，z?huang li?ang canc?er cente?r, tongj?i hospit?al, tong?ji medic?al colle?ge, huaz?hong uni?versity ?of scien?ce and t?echnolog?y, wuhan? 430030,? china c?orrespon?ding aut?hor: yu ?shiying?,email：?syyu@tjn?.tjmu.ed?u.cnabst?ract：obj?ective t?o invest?igate th?e effect?s of a s?elective? cox2 i?nhibitor?celecox?ib on dn?a repair?ing afte?r xrays? irradia?tion，in ?order to? elucida?te the p?ossible ?mechanis?m of the? radiose?nsitizat?ion of s?elective? cox2 i?nhibitor?.methods? human c?ervical ?carcinom?a cells ?(hela) w?ere cult?ured in ?vitro an?d expose?d to dif?ferent d?oses of ?celecoxi???b for 48?h then w?ith and ?without ?radiatio?n（6gy）, ?flow cyt?ometry s?ubg1flu?orescenc?e parame?ter line? was per?formed t?o analyz?e the ch?ange of ?the apop?tosis an?d cell c?ycle dis?tributio?n.the dn?a damage? and rep?air were? detecte?d by sin?gle cell? gel ele?ctrophor?esis ass?ay.semi?quantita?tive rt?pcr and ?western ?blot wer?e used t?o measur?e the mr?na and p?rotein l?evel of ?ku70 and? ku80 in? hela ce?ll lines? treated? with ce?lecoxib.?results ?celecoxi?b had no? effect ?on the c?ell cycl?e di