健膝丸含药血清对il-1β作用下关节软骨细胞表达type-ⅱcollagen mmp-1 mmp-13的影响-effect of jiangqi pill containing serum on type - ⅱ collagen mmp - 1 mmp - 13 expression in articular chondrocytes under il - 1β.docxVIP

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健膝丸含药血清对il-1β作用下关节软骨细胞表达type-ⅱcollagen mmp-1 mmp-13的影响-effect of jiangqi pill containing serum on type - ⅱ collagen mmp - 1 mmp - 13 expression in articular chondrocytes under il - 1β.docx

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健膝丸含药血清对il-1β作用下关节软骨细胞表达type-ⅱcollagen mmp-1 mmp-13的影响-effect of jiangqi pill containing serum on type - ⅱ collagen mmp - 1 mmp - 13 expression in articular chondrocytes under il - 1β

英文缩写词表Type-ⅡCollagenⅡ型胶原MMP-1、基质金属蛋白酶 1MMP-13基质金属蛋白酶13摘要目的：观察健膝丸含药血清对 IL-1β 作用下体外培养的兔关节软骨细胞表达 Coll-Ⅱ、MMP-1、MMP-13的影响，探讨健膝丸防治OA的作用机制。明确“健膝丸” 治疗KOA的作用靶点和影响途径，为临床用药提供循证医学证据，丰富膝关节骨性关节炎的治疗药物，为临床推广健膝丸提供基础。方法：获取1月龄兔关节软骨细胞，取第Ⅰ代细胞用于实验。实验分为空白组，模型组、健膝丸组、抗骨增生组4组，培养第4天时，模型组、健膝丸组及抗骨增生组分别加入IL-1β 10ng/ml继续培养，分别在培养后24h、48h、72h用In-cell western blot法检测各蛋白表达情况。结果：各时间点空白组与模型组Coll-Ⅱ、MMP-1及MMP-13表达差异均有显著性（P＜0.05），骨痹舒组较模型组Coll-Ⅱ表达在48h、72h时间点有显著性差异（P＜0.05），MMP-1及MMP-13表达在各时间点均有显著性差异（P＜0.05）。结论：骨痹舒能有效抑制 IL-1β 对Coll-Ⅱ 的降解和破坏作用，能抑制 IL-1β对 MMP-1、MMP-13的诱导和激活作用。关键词：软骨细胞健膝丸含药血清Ⅱ型胶原基质金属蛋白酶Effects of Jianxiwan containing serum on the expression of articular chondrocytes Type-ⅡCollagen、MMP-1、MMP-13 stimulated by interleukin-1betaZhaomin(Rheumatism) Directed by Lou Yu-qianAbstractObjective: To investigate the effects of Jianxiwan containing serum on the expression of Coll-Ⅱ、MMP-1、MMP-13 of rabbit articular chondrocytes stimulated by interleukin-1beta. Method: Articular chondrocytes were obtained from the cartilage of 1-month rabbit and culturedin vitro. The passageⅠchondrocytes were used. They were divided into 4 groups, controlgroups、model groups、Jianxiwan groups and Kangguzengsheng groups. After 4 days cultured, IL-1β 10ng/ml were added in the control groups、Jianxiwan groups and Kangguzengsheng groups.In cell western blot assay method was adopted to observe the influence of every different groups on the expression of Coll-Ⅱ 、 MMP-1 and MMP-13 of chondrocytes stimulated by interleukin-1beta after incubated 24h、48h and 72h.Result: The expression of Coll-Ⅱ、MMP-1and MMP-13 of chondrocytes were significant differences between control groups and model groups at same time point (P＜0.05); The expression of Coll-Ⅱ of chondrocytes were significant differences between Gubishu groups and model groups at 48h、72h (P＜0.05); There was significant differences in expression of MMP-1and MMP-13 at same time point(P＜0.05). Conclusion: Jianxiwan containing serum can signif