高浓度胰岛素对人树突状细胞分化成熟及免疫功能影响.docVIP

高浓度胰岛素对人树突状细胞分化成熟及免疫功能影响 　　中图分类号：R446 文献标识码 ：A 文章编号：1009_816X(2010)02_0100_03 　　 Effects of Hyperinsulin on the Development and Maturation and Immune Function ofHuman Derived Dendritic Cells. CHEN Li_ping, YE Shuang_ying, WU Rong_zhen, et al. Lishui Center Hospital, Zheji ang 323000, China 　　［Abstract］ ObjectiveTo explore the effect of hyperinsulin on the maturation ofmonocyte_derived dendritic cells (DC).MethodsHuman monocytes were purifed usi ng successive adherence method, after 5 days culture in RPMI1640 medium contain ing granulocyte_macrophage colony stimulating factor （GM_CSF，100ng/