医学PPT课件大全New Research Field in Molecular .ppt

1. What is Proteome? Proteome - All the proteins expressed by a cell , tissue or organism at a particular time and under specific conditions. 4. Branches of Proteomics Expressional Proteomics Functional Proteomics Structural Proteomics Protein Microarrays 蛋白质芯片的意义 用于构建蛋白质表达谱, 进行抗原-抗体筛选, 药物靶点筛选, 蛋白质-蛋白质交互作用筛选等 是蛋白质组进行功能分析的新技术 一种蛋白质的所有变异体 一个蛋白质家族的所有成员 一条信号通路中的所有蛋白质 一种组织、器官或有机体的所有蛋白质 Different Kinds of Protein Arrays 蛋白质芯片检测 蛋白质芯片的应用 ?疾病诊断和预警 ?药物开发 ?蛋白质组学 Problems Expensive Standard Specificity Sensitivity Functional Proteomics (in vitro) In silico methods (bioinformatics) Genome-wide Protein Tagging Genome-wide Gene Deletion or Knockouts Random Tagged Mutagenisis or Transposon Insertion Yeast two-hybrid Methods Protein (Ligand) Chips Structural Proteomics Structural Proteomics: The Goal Why Structural Proteomics? Bioinformatics Proteomics 代谢组学的应用 疾病诊断 药物的疗效和毒性评价 植物的细胞代谢组学评价 By the end... * * Chapter 8 New Research Field in Molecular Biology 8.1 Bioinformatics What is bioinformatics? Bioinformatics and computational biology involve the use of techniques including applied mathematics, informatics, statistics, computer science, artificial intelligence, chemistry, and biochemistry to solve biological problems usually on the molecular level. 2. Major research fields (1) Sequence analysis (3) Computational evolutionary biology (4) Measuring biodiversity (5) Analysis of gene expression (6) Analysis of regulation (7) Analysis of protein expression (8) Analysis of mutations in cancer (9) Prediction of protein structure (10) Comparative genomics (11) Modeling biological systems (12) High-throughput image analysis 3. Databases ?BLAST ACCESS ?dbEST / Expressed Sequence Tags database ?ceDB databases ? Phenotypic databases ?LIMS ?BASE ?MOLCAS …….. 8.2 Proteome Proteomics Proteomics is the systematic study of all of the proteins in a cell, tissue, or organism. The analysis includes the identi