蛋白质研究的其他方法PPT.ppt

Rta promoter (Rp) （转录brlf1）is a direct target for activation by ZEBRA. CMV, (p) empty plasmid; Chromatin immunoprecipitation of ZEBRA protein and its associated DNA.(检测时要带上之前取出的部分原液作为Input对照) BZKO cells were transfected with expression vectors encoding wt ZEBRA, a non-DNA binding mutant Z(R183E), and three replication defective ZEBRA mutants [Z(Y180E), Z(R187K) and Z(K188A)]. PAA was added to block viral DNA replication. Cells were harvested 48 h after transfection. （B) Relative amount of oriLyt DNA either present in input samples or immunoprecipitated by pre-immune（没有加蛋白） or anti ZEBRA an