抗生素PPT课件（英文精品）Quality control（QC） of antibiotic susceptibility .pptVIP

Quality control(QC) of antibiotic susceptibility testing The routine laboratory A culture of QC is essential Other Essential ingredients The Commitment Value of antibiotic QC For the patient The laboratory The Hands and Heads Skilled personnel The media The incubator The bug The antibiotics The standard operating procedures (SOP) Commitment – recognising the value of QC To monitor and ensure the accuracy and precision of our antibiotic susceptibility testing Allows us to rely on the reproducibility and accuracy of our daily results Allows communication of accurate and meaningful results to the clinician The hands and heads - personnel Essential that everyone is aware of the importance of QC Training of personnel in correct technique Storage of discs Preparation of a standard inoculum Swabbing of plates Choice and storage of media Timing and methods of incubation of plates Measurement of zone sizes Recording of results The Media essentials Thickness pH Cations Magnesium Calcium Storage Dry before use At room temperature before use Incubator Knowledge of requirements for incubation Ambient air at 350C for non fastidious aerobic and facultative anaerobic bacteria Timing – generally 16-18 hours 24 hours for S.aureus and enterococci 5% CO2 for Haemophilus Stacking of plates No more than 5 plates high The bug ATCC strains Correctly stored Sub-cultured regularly Pure culture Correct inoculum preparation Timing of swabbing within 15 minutes of preparing inoculum Antibiotic discs Stored and handled correctly Refrigeration – taken out 1 hour before use Expiry dates noted Discs at room temperature before use Avoid condensation Placing of discs within 15 minutes of swabbing Decide on your procedure Who will do it? How often? What does the NCCLS say? Daily Weekly Who will assist with troubleshooting? Designate an individual How will problems be communicated to clinicians When? When things go wrong… Questions to ask? Is the procedure correct? Che