抗生素英文课件精品KirbyBauer .pptVIP

Kirby-Bauer Test Testing for Antibiotic Resistance Why bother testing? K-B Test is routinely done to monitor the prevalence of antibiotic resistant bacteria Observe for a trend in order to take precautionary measures For example: development of new drugs determining the molecular basis for resistance and modify existing drugs accordingly Procedures Prepare a pure culture (18-24 hrs) of the sample on a non-selective medium Adjust turbidity until it is equivalent to the 0.5 McFarland Turbidity Standard Procedures Within 15 minutes of adjusting the turbidity dip a sterile cotton swab into the sam