层析分离纯化技术幻灯片.pptVIP

* * * * * * Picture. Show the tree different sizes of SOURCE media on the market. The SOURCE family consist of 30, 15 and now also 5um particles. 30 um media that is the largest particle in the family has been modified for ion exchange chromatography (strong Cation and Anion) as well as for RPC. This media size is used in process chromatography. The 15 um particle that has been on the market the longest period of time is being modified with HIC ligands as well. HIC phenyl as the most hydrophobic HIC ether as the most hydrophilic and HIC isopropropyl with a hydrophobicity in-between. The latest product in the SOURCE family is an analytical 5um RPC particle, which can be used as an analytical RPC column for protein fragment, peptide mapping and oligonucleotides analysis. Lowest curve Gives an indication of peak width achieved with particles of different diameter. The reason for band broadening is longer diffusion distance in a large particle compared to a small particle, but it is also a matter of technique * This is an attempt to describe where the individual chromatography techniques fit best in a purification procedure. Each techniques’ advantages and disadvantages were taken into account. Comment on the star-rating for GF: Note that a desalting step can sometimes be an appropriate initial step in a purification scheme. But since GF is a diluting technique, it does not merit any sars as a true capture technique. Under “considerations” is listed the “negative” aspects (if any!) of the different techniques. THE ABBREVIATIONS GF, IEX, HIC, AC and RPC SHOULD BE EXPLAINED! These abbreviations (together with AIEX and CIEX) are used throughout the remainder of the lecture. * This is an attempt to describe where the individual chromatography techniques fit best in a purification procedure. Each techniques’ advantages and disadvantages were taken into account. Comment on the star-rating for GF: Note that a desalting step can sometimes be an appropriate initial