研究生病理学总论血栓性疾病课件.ppt

Immunohistochemical staining of EPCR in human spleen and peripheral artery. A, EPCR stains moderately to strongly in a follicular arteriole (arrow) and moderately in the venous sinusoids in this autopsy case. Negative to weak EPCR staining in the venous sinusoids was seen in other autopsy cases and in the baboon spleen. B, The popliteal artery reveals strong EPCR staining. Note that the smooth muscle cells of the arterial wall and the surrounding connective tissue are negative. Immunohistochemical staining of EPCR in normal baboon tissues. Paraffin-embedded tissues were incubated with the anti-EPCR mAb 1489 (A through J). Bound antibody was detected with streptavidin peroxidase methodology as described in “Methods.” A, Low magnification of the heart showing strong staining of the veins, some postcapillary venules, and the endocardium of the left ventricle (arrow). B, Heart: Higher magnification of EPCR staining within postcapillary venules. The capillaries are negative. C, Heart: A higher magnification of a large coronary artery (left anterior descendent) shows strong staining in all endothelial cells. D, Brain: Subarachnoid vessels with positive EPCR staining are seen in the frontal cortex with negative to weak staining of the capillary endothelial cells. E, Kidney: Positive endothelial staining is present in an interlobular artery. Note that the glomerulus (arrow) and the cortical tubulointerstitial capillaries are negative for EPCR expression. F, Kidney: Endothelial cells of the vasa recta are moderately EPCR positive in the renal medulla. G, Adrenal cortex: EPCR staining is seen in the capillary endothelial cells in the zona fasciculata and reticularis. H, Endometrium: Strong endothelial EPCR staining is present in a spiral artery (arrow) of the endometrium. Note that the capillary endothelia are either negative or stain weakly. Scheme of the coagulation-anticoagulation systemActivation of the coagulation system culminates in generation of thrombin, which cleave