Haloferax sulfurifontis sp nov, a Halophilic Archaeon Isolated 嗜盐sulfurifontis sp. nov.，嗜盐古菌分类.pptVIP

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2018-06-15 发布于福建
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Haloferax sulfurifontis sp nov, a Halophilic Archaeon Isolated 嗜盐sulfurifontis sp. nov.，嗜盐古菌分类.ppt

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Haloferax sulfurifontis sp nov, a Halophilic Archaeon Isolated 嗜盐sulfurifontis sp. nov.，嗜盐古菌分类

Haloferax sulfurifontis sp. nov., a Halophilic Archaeon Isolated from a Low Salt Sulfide-rich Spring K. N. Savage1, M. S. Elshahed1, A. Oren2, L. R. Krumholz1 1University of Oklahoma, Norman, OK, 2The Hebrew University of Jerusalem, Jerusalem, ISRAEL Zodletone Spring /states/maps1/ok.gif 8 l?min-1 20 m from source to creek High sulfide concentrations (8-10mM) 0.2 M NaCl F, Br, Li, B, Sr, Ba, SO42- BaSO4 and CaCO3 Dissolved sulfur Sulfur Cycling S2- S° SO42- H2S H2S H2S H2S H2S H2S H2S H2S H2S SO42- SO42- SO42- SO42- SO42- Sulfur Cycling H2S H2S H2S H2S H2S H2S H2S H2S H2S SO42- SO42- SO42- SO42- SO42- S° HS- SO42- Sulfur Disproportionation So HS- SO42- Archaeal Diversity Halophilic Archaea 14 validly described genera Isolated from hypersaline environments (i.e. The Dead Sea) Rods, cocci and pleomorphic forms Require high salt concentrations from 3.5 M NaCl to saturation (5.2 M) Contain pigment material (bacteriorhodopsin and halorhodopsin) which gives them a distinct pink or red color Isolation of Haloarchaea 36% of the mat and 4% of the source library Isolated halophiles from the mat using a high salt plus antibiotic medium with different salt concentrations (7, 12 and 18%) Serially diluted mat material and plated onto HM plates (immediately and after incubation) Cultures were incubated at 37oC under light A Novel Species of Haloferax 16S rRNA sequence analysis of strain M6 showed a 96.7-98.0% similarity to other validly described species of this genus M6 was 89% similar to Halogeometricum borinquense the most closely related species outside the genus DNA-DNA hybridization confirmed its novel speciation having a hybridization value of only 24% to Haloferax gibbonsii Phylogeny Haloferax sulfurifontis Isolated from Zodletone spring Extreme pleomorphism Colonies were small (2-3 mm) and salmon pink in color at 37°C Growth occurred in a wide range of salt concentrations (6% to saturation) Required at least 1 mM Mg2+ for growth Anaerobically reduces So Haloferax su