FOURIER TANSFORM INFRARED傅里叶变换红外.docVIP

Analysis Improvement of Drugs Using Modern Methods A. A. BUNACIU*, S. FLESCHIN**, H. Y. ABOUL – ENEIN*** *O.F. Systems - Mettler Toledo AG. Analytical Expertise Department, 70644 - 25 Str. Al. ?erb?nescu, PO Box 52 - 3 , Bucharest , ROMANIA **University of Bucharest, Department of Analytical Chemistry, 76229 – 90-92 ?os. Panduri , Bucharest, ROMANIA ***King Faisal Specialist Research Centre, Bioanalytical Drug Development Laboratory, PO Box 3354 Rijadh, SAUDI ARABIA Received: 28th December, 2001; Accepted: 29th January, 2002 Abstract A Fourier transform infrared (FT-IR) spectrometric method (DRIFTS) was developed for the rapid, direct measurement of mebendazole in drugs. Conventional KBr-spectra and DRIFTS-spectra were compared for best determination of active substance in drug preparations. Two chemometric approaches, partial least-squares (PLS2) and principal component regression (PCR+) methods were used in data procesing. The best results were obtained with PLS2 method. Keywords: fourier transform infrared spectrometry, polymorphic analysis, mebendazole drug analysis, chemometric analysis. Introduction Mebendazole, (Meben) { methyl - ( 5-benzoyl-1H-benzimidazol-2yl ) carbamate} is a synthetic broad – anthelmintic drug, active against nematodes and cetodes1,2, being indicated in the treatment of single or mixed infestation by Enterobius vermicularis, Trichuris trichiura, Ascaris lumbricoides, Ancylostoma duodenale, Necator americanus, Strogyloides stercoralis, Taenia app. in human and veterinary therapeutics3. Polymorphism is a technical term used both in biology and crystallography, describing the fact that a natural phenomenon may occur in two or more different forms. Polymorphs are the same chemicals with different molecular arrangements within the crystal lattice. In principle, the different polymorphic forms of a compound have different energies and this could affect their bioavailability. Sometimes there are more than two forms. Meben used in clinical