内皮祖细胞的研究进展课件.pptVIP

Bone marrow Histopaque 1083 Methods Mononuclear cells (MNCs) were isolated from mouse bone marrow with Histopaque 1083 by density gradient centrifugation. Bone marrow Histopaque 1083 Resuspend in EGM2 Plate 5X106 cells/well on fibronectin-coated 6-well dish Methods MNCs were cultured in EGM2 with the supplement of VEGF, b-FGF, IGF-1 on fibronectin-coated 6-well plate for 2 days to remove mature endothelial cells and monocytes. Bone marrow Histopaque 1083 Resuspend in EGM2 Plate 5X106 cells/well on fibronectin-coated 6-well dish 2 days Collect no-adherent cells Plate 1X106