纤支镜毛刷细胞块技术应用方法和价值.docVIP

纤支镜毛刷细胞块技术应用方法和价值 　　[摘要] 目的 探讨纤支镜毛刷细胞块技术的应用方法和价值。 方法 对32例纤支镜毛刷常规涂片后再将剩余细胞用10%中性福尔马林固定制成细胞蜡块（cell block，CB），先行常规HE染色，再根据需要选择酶标行免疫组化。 结果 CB常规HE染色细胞清晰，有一定的组织学结构，有利于明确诊断；免疫组化则阳性定位准确，着色清晰，对肿瘤分型效果较好。两种染色方法染色效果均好于常规涂片，差异有显著统计学意义（P 0.05）。 结论 用10%中性福尔马林固定的纤支镜毛刷CB的制作方法简便、经济、染色效果好，在诊断肺疾病、特别是肺肿瘤中有较高的实用价值和广阔的应用前景，值得推广。 　　[关键词] 纤支镜毛刷；细胞块；常规HE染色；免疫组化；10%中性福尔马林 　　[中图分类号] R446.6；R361.3 [文献标识码] B [文章编号] 1673-9701（2013）36-075-03 　　The application methods and value of fiber lens brush cell block technique 　　ZHANG Guizhen ZHU Zhenda CHEN Cheng 　　Department of Pathology， Yuhang District Traditional Chinese Medicine Hospital of Hangzhou City in Zhejiang Province， Hangzhou 311106， China 　　[Abstract] Objective To study the application methods and value of fiber lens brush cell block technique. Methods For 32 cases of fiber lens brush after conventional smear in the remaining cells again fixed with 10% formaldehyde wax block made from cells （cell block， CB）， the first routine HE staining， again to choose according to the need to enzyme immunohistochemical marking line. Results CB routine HE staining cells clear， histologic structure to a certain extent， was conducive to clear diagnosis； Immunohistochemical positive for accurate positioning， color clear， has good effect for tumor classification. Two dyeing methods were better than regular smear dyeing effect， so the significant difference of statistically was significant （P 0.05）. Conclusion The production method of the fiber lens brush CB fixed with 10% of formaldehyde is simple， economic and The dyeing effect is good， in the diagnosis of pulmonary diseases， especially lung cancer has a high practical value and broad application prospect， the production method is worth promoting. 　　[Key words] Fiber lens brush； Cell block； Routine HE staining； Immunohistochemical； 10% formaldehyde 　　纤支镜毛刷细胞学检查是诊断肺疾病、特别是肺肿瘤的重要手段之一，已被广泛用于临床，当纤支镜活检和经皮肺穿刺活检由于多种因素被迫放弃，此时的纤支镜毛刷细胞学检查是术前最佳的病理检查方法，显得尤为重要，并对已不适合手术治疗的老弱患者和晚期癌症患者来说也是较佳的病理检查方法。笔者对32例纤支镜毛刷常规涂片