萝卜硫素对大鼠局灶性脑缺血·再灌注损伤神经元保护机制.docVIP

萝卜硫素对大鼠局灶性脑缺血·再灌注损伤神经元保护机制 　　【摘要】目的研究萝卜硫素（SFN）对大鼠局部脑缺血-再灌注损伤的神经保护机制。方法24只雄性SD大鼠，随机（随机数字法）分为3组，A组：假手术组（n=8），B组：缺血-再灌注模型组（n=8），C组：萝卜硫素干预组（n=8）；A组：假手术后腹腔注射等量磷酸盐缓冲液（PBS），B组：缺血-再灌注术后腹腔注射等量PBS，C组：缺血-再灌注术后腹腔注射萝卜硫5 mg/kg。通过四氮唑红（2，3，5-Triphenyltetrazolium chloride，TTC）染色测定脑梗死体积，苏木精伊红染色法（HE）观察组织学形态改变，TUNEL 法检测脑神经元凋亡变化，RT-PCR 测定缺血脑组织核转录因子kappa B亚单位p65（NF-κBp65）、诱导型一氧化氮合酶（iNOS）mRNA的表达，免疫蛋白印记法（Western-bloting）检测脑组织iNOS、NF-κBp蛋白表达水平。结果与B组比，C组脑梗死体积降低 [（96.34±3.72） vs. （124.65±3.85），P 　　【关键词】萝卜硫素；缺血-再灌注；损伤；神经元；凋亡；TUNEL；核转录因子κB；诱导型一氧化氮合酶 　　Protective effects of sulforaphen on neuronal apoptosis after focal cerebral ischemia reperfusion injury in rats ZENG Wei-xian，CHEN Da-qing，GONG Yu-qiang，SUN Lai-fang，LU Zhong-qiu. Department of Emergency Medicine，The Second Affiliated Hospital of Wenzhou Medical College，Wenzhou 325000，China 　　Corresponding author：CHEN Da-qing， Email： cdq1965@126.com 　　【Abstract】ObjectiveTo investigate the protective effects of sulforaphen （SFN） on focal cerebral ischemia/reperfusion injury （IRI） in rats in order to explore the mechanisms. MethodsTwenty-four male SD rats were randomly（random number） divided into Sham-operated group （A group， n=8）， IRI group （B group， n=12）， sulforaphen group （C group， n=8）. SD rats were made to be transient focal cerebral IRI models. SFN 5 mg/kg was injected intraperitoneally to rats 15 minutes after IRI in C group， and rats of group A and group B received equal volume PBS instead. Infarct volume was measured by TTC staining and morphologic changes were observed with HE staining. Neuronal cell apoptosis index was detected by terminal deoxynucleotidyl transferase （TdT）-mediated dUTP-biotin nick end labeling （TUNEL） assay. Rats were sacrificed at 24 h after IRI. The protein levels of NF-κB p65 and iNOS were detected by using western bloting and the mRNA expressions of NF-κB p65 and iNOS were detected by using RT-PCR.ResultsCompared with the group B， infarct volume was significantly smaller in group C， the number of neuronal cel