转录因子GATA—3和T—bet表达失衡在复苏后急性肺损伤中作用.docVIP

转录因子GATA—3和T—bet表达失衡在复苏后急性肺损伤中作用 　　【摘要】目的 探讨转录因子GATA-3和T-bet的表达失衡是否参与了猪心肺复苏后的急性肺损伤机制。方法 26头猪随机（随机数字法）分为假手术组（SHAM）（6只）、复苏12 h组和复苏24 h组（各10只）。建立室颤8 min小型猪模型；用流式细胞术检测猪外周血淋巴细胞（PBL）中CD3+、CD4+和CD8+淋巴细胞亚群的分布；采用酶联免疫吸附（ELISA）方法检测血浆中白细胞介素-4（IL-4），肿瘤坏死因子α （TNF-α）和干扰素γ（INF-γ）的含量；采用Western-blot法检测肺组织GATA-3和T-bet的蛋白表达；用实时荧光PCR法定量肺组织GATA-3和T-bet mRNA水平。结果 复苏组小猪发生严重肺组织细胞损伤，在复苏后的12 h和24 h，猪血清中CD4+细胞的比例（28，4±2，3）%，（24，1±1，6）%明显低于假手术组（48，4±2，9）%，（51，1±5，4）%；CD4+/CD8+（1，7±0，9），（1，5±1，0）也较假手术组明显减低（2，5±1，3），（2，7±1，1）（P 　　【关键词】心肺复苏；急性肺损伤；Th1/Th2失衡；免疫功能障碍；GATA-3；T-bet 　　Role of imbalance between transcription factors GATA-3 and T-bet expressions in the pathogenesis of acute lung injury after resuscitation Gu Wei，Li Chunsheng，Yin Wenpeng，Zhang Jian，Hou Xiaomin，Zhang Da. Department of Emergency Medicine， Beijing Chaoyang Hospital， Capital Medical University， Beijing 100020， China 　　Corresponding author： Li Chunsheng， Email： banditgu@hotmail，com 　　【Abstract】Objective To study the role of imbalance between transcription factors GATA-3 and T-bet expressions in causing acute lung injury after resuscitation in cardiac arrest model of swine.Methods After swine model of electrically induced cardiac arrest was established for 8 minutes， animals were resuscitated to get restoration of spontaneous circulation （ROSC）. The swine with ROSC were randomly assigned to be sacrificed at 12 and 24 h after ROSC （n=8 in each group）. CD3+，CD4+ and CD8+ lymphocyte subsets were determined by flow cytometry， and the levels of serum IL-4，TNF-α，and IFN-γ were measured by using ELLSA. The protein levels and expressions of GATA-3/T-bet mRNA were detected in lung tissue by western blotting and quantitative real-time PCR device， respectively.Results Pulmonary function was significantly impaired after ROSC. CD4+ lymphocyte subsets???28，4±2，3）%，（24，1±1，6）%and CD4+/CD8+ （1，7±0，9），（1，5±1，0）were significantly lower in the post-ROSC group compared with the sham-operated group（48，4±2，9）%，（51，1±5，4）%（2，5±1，3），（2，7±1，1） （P