Virus vectoring ability of a Xiphinema americanum - Oregon State 病毒矢量的剑线虫americanum -俄勒冈国家能力.pptVIP

Virus vectoring ability of a Xiphinema americanum - Oregon State 病毒矢量的剑线虫americanum -俄勒冈国家能力.ppt

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Virus vectoring ability of a Xiphinema americanum - Oregon State 病毒矢量的剑线虫americanum -俄勒冈国家能力

By: Charlie Ta Mentor: Dr. Inga Zasada United States Department of Agriculture: Agricultural Research Services Plant-parasitic nematodes cause $100 billion in crop loss annually worldwide; $10 billion in the U.S. (blueberries, red raspberries, and wine grape industry) Plants affected by X. americanum or nepoviruses become(s) necrotic, yield is reduced, and plant mortality can occur Currently few methods exist to control nematodes or remediate the diseases they transmit Regulations by the U.S. Environmental Protection Agency will soon limit/ban pre-plant fumigation which has traditionally been used to eradicate virus-transmitting nematodes Microscopic roundworm(s) that parasitize plants Migratory ectoparasite Acquire and transmit nepoviruses such as Tomato Ringspot Virus (ToRSV) and Tobacco Ringspot Virus (TRSV) with their odontostyle head tail Nematode-transmitted virus with polyhedral particles Type IV virus under the Baltimore classification system (positive sense single-stranded RNA that directly translates into protein) Acquisition of virus occurs during feeding and binds to the surface of the odontostyle Viruses are lost when nematodes molt odontostyle A RT-qPCR can be used for the detection of ToRSV in X. americanum at low concentration levels. Virus detection using RT- qPCR allows for a detailed study of nematode-virus interactions. The coloration occurs due to adding p-nitrophenyl phosphate. http://homepage.usask.ca/~vim458/virology/studpages2007/Maura_Tim/For%20Maura%20-%20Virology%20website%20assignment/elisa.jpg 1 2 3 4 5 Steps: Reverse Transcriptase -Quantitative Polymerase Chain Reaction (RT-qPCR) Enzyme-Linked Immunosorbent Assay (ELISA) Could a RT-qPCR method be developed to enable detection of small concentrations of ToRSV? A RT-qPCR method will be proficient in detecting low concentrations of viruses. X. americanum acquires ToRSV within a week of feeding on a virus infected host. This time period allows for additional virus particles to be ac

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