Reactive Crystallization of Calcium Hydroxyapatite - Advanced 反应结晶羟基磷灰石-高级.ppt

* * Biomedical Research Reactive Crystallization of Calcium Hydroxyapatite Chen-Lu Yang Advanced Technology and Manufacturing Center University of Massachusetts – Dartmouth Alison Mello Department of Chemistry and Biochemistry University of Massachusetts – Dartmouth Dapeng Li Department of Materials and Textiles University of Massachusetts – Dartmouth Introduction: In recent decades, a considerable amount of research has focused on developing biomaterials that can both positively react and replace bone and its derivatives including dentin and other hard tissues found in teeth. One such bioceramic is hydroxyapatite (HAP), a calcium phosphate. Because HAP has been shown to possess high biocompatibility while causing little or no damage to existing host tissue, it is currently used in dentistry and medicine to remodel bone and as the base or surface coating for a variety of tooth, bone, and orbital implants. Because the exact processes of bone formation and differentiation remain unknown, an understanding of the properties of its synthetically derived compliments may be valuable in better mimicking and manipulating the elusive biochemical mechanisms in vivo. Materials and Methods: To prepare all aqueous hydroxyapatite crystals in this procedure, 1.0 M calcium hydroxide and 0.6 M phosphoric acid were reacted in a wet crystallization procedure. Although other calcium and phosphate reactants will have been shown to produce functional HAP, the products of the simple acid-base reaction below are limited to HAP and water, a biologically non-damaging compound. Crystal analysis was accomplished using a JEOL JSM 5610 scanning electron microscope (SEM), and energy depressive X-ray (EDX) analysis was done using an Oxford 6587 instrument. 10 Ca(OH)2 + 6 H3PO4 ? Ca10(PO4)6(OH)2 + 18 H2O (SEM EDX analysis) In all procedures, the calcium hydroxide and phosphoric acid solutions were reacted in stoicheiometric volumes so that all of the calcium hydroxide reacted while li