Sterilization Device for Liquid Chromatography Solvents液相色谱溶剂灭菌装置.pptVIP

Sterilization Device for Liquid Chromatography Solvents Design Team Nick Roulleau, Michael Vose Michael Racette, Michael McKay Introduction Background Problem Statement Past Art Design Requirements Design Concepts Prototype Design Component Analysis Recommendations What is Liquid Chromatography? Basic Components of an HPLC System Design Goal To mitigate the risk of blockage at the inlet frit due to bacterial contamination and extend the useful life of the UPLC column. Existing Solutions In-line filters Guard columns and cartridges Product Requirements Mandatory: Must be adaptable for use worldwide Must extend the useful life of columns Must meet safety standards (ISO, UL and CE) Must operate for 1 year w/o user intervention Desirable: Should be able to filter two bottles simultaneously Should meet customer acceptance criteria Low-maintenance Easy to use Cost Constraints Cannot change the chemical composition Of the solvent Of the sample Cannot create risk of causing pump cavitation Cannot hinder bottle accessibility Cannot negatively impact system resolution Initial Design Concepts Preliminary Design – UV Probe Why Not Use Ultraviolet Radiation as a Primary Solution? Degradation of organic solvent modifiers (Low Risk) Degradation of aqueous additives (Low Risk) User safety from UV-C exposure (Medium Risk) UV can inactivate but not remove bacteria Filter Sizing How many bacteria could be generated per year? Logarithmic growth: Assuming worst case 100% replicating Short generation time Neglecting lag phases and cell death Filter capacity = 107 CFU/cm2 Filter Sizing With logarithmic bacterial growth, filter area becomes exceptionally large in a short period Current DesignExternal Filter Enclosure with UV Filter Selection Membrane with material compatibility Sufficient capacity to contain 1 year of inactivated bacteria Pump Selection Micro-diaphragm pump Dual pump heads Ability to run dry DC brushless motor for long life Pump Pressure