拉曼光谱成像中的图像构算法研究.docVIP

毕业论文 （本 科 生） 论文题目（中文）论文题目（外文）Algorithm based on Raman Spectroscopy 拉曼光谱成像中的图像重构算法研究 摘 要 拉曼散射是一种特殊的光散射现象，光的频率经过散射后会发生变化，频率的变化取决于散射的物质。对于相同频率的入射光，不同的分子，原子团，官能团会散射特定频率的光，其光谱是基于拉曼散射效应的分子光谱，以光子为探针反映物质内部不同分子间的振动关系，通过对它光谱的分析，由光谱构建图像，可以得到散射物质的各种化学成分的种类和它的空间分布。 本课题中，实验数据是激发光源为532nm，分辨率为32*32的细胞拉曼散射光谱图，通过选定554nm处的特征峰作为目标，将554nm处的特征峰的幅度值提取计算,然后把幅度值映射为RGB分量值。考虑到实验数据分辨率只有32*32，最后会将图像放大10倍显示，图像放大中的插值算法采用双线性插值算法，在MATLAB上完成图像显示，从而反映在细胞内的分布情况。 关键词：拉曼散射，图像重构，双线性插值算法 RESEARCH ON IMAGE RECONSTRUCTION ALGORITHM BASED ON RAMAN SPECTROSCOPY Abstract Raman scattering is a special light scattering phenomenon. The frequency of scattered light is dependent on the materials and will be different from the incident beam. With the same laser excitation, various molecules, atoms and functional groups present specific scattering wavelength. The Raman spectrum, which is molecular spectroscopy based on vibration probe, reflects information of different molecules of material. Through the analysis of Raman spectrum and imaging construction based on the Raman spectrum of the sample, the chemical composition and its spatial distribution can be obtained. In this paper, the experimental data is obtained under the excitation laser 532nm with the resolution of 32*32 cells. The characteristic peak at 554nm is selected as the target, the amplitude of the characteristic peak is extracted, and the amplitude value is mapped to RGB component value. Considering the experimental data, the resolution is only 32*32, finally the image is magnified by 10 times to show that the interpolation algorithm in image zooming using bilinear interpolation algorithm, complete the image displayed on the MATLAB, so as to reflect the distribution within the cell. Key words: Raman scattering，Image reconstruction，bilinear interpolation目 录 摘 要 I Abstract II 一 绪论 4 1.1 拉曼效应的发现和发展 4 1.2 拉曼效应的应用 5 1.3 选题背景和意义 5 1.4 双线性插值 6 1.5课题的主要内容 8 二 课题研究步骤 9 2.1 特征峰幅度值数据提取 9 2.2 数据向RGB分