医学组织芯片简易制作法及免疫组化有效性分析.docVIP

组织芯片简易制作方法及免疫组化有效性分析 作者：漆楚波，朱润庆，夏和顺，王明伟 【摘要】 目的探索一种组织芯片的简易制作方法，并对它的有效性进行分析。方法在实验中探索出组织芯片的制作方法，收集乳腺癌病例124例，制作成直径1.6cm的组织芯片，进行雌激素受体（ER）、孕激素受体（PR）及cerBb2 受体免疫组化染色，同时与普通切片的免疫组化染色结果对比，并进行统计学分析。结果组织芯片与普通切片的ER、PR及cerBb2 染色积分（0 ～ 7 分）显著相关，ER、PR 及cerBb2的表达（阳性/ 阴性）一致率分别达94.35%、93.55%和91.94%。比较组织芯片与普通切片的ER、PR及cerBb2的表达，两者之间无统计学差异（Pgt; 0.05），即组织芯片的结果与普通切片的结果是一致的。结论自制1.6mm 直径的组织芯片在进行免疫组化染色时能够代表普通切片,自制组织芯片的方法可靠有效。 【关键词】 组织芯片；简易制作方法；雌激素受体；孕激素受体；cerBb2受体；免疫组化 　　A Simple Making Method of Tissue Microarray and Validation Study on Immunohistochemistry 　　Abstract：ObjectiveTo search a simple making mothd of tissue microarray and evaluate its validation. MethodsA new, simple and easy method was designed to improve the technology of TMA during the experiment. Tissue microarrays with 1.6mm in diameter tissue core were constructed from 124 cases of breast cancer. The tissue microarrays were stained for oestrogen receptor（ER）,progesterone receptor（PR）and cerBb2 using immunohistochemistry.At the same time the stains on the tissue microarrays were compared with that from the full tissue sections. ResultsA highly significant association was observed between the staining scores（0 ～ 7）obtained from the full tissue sections and from the tissue microarrays. Concordance for the receptor status（positive / negative）of the whole section and the tissue core were 94.35% for ER，93.55% for PR.and 91.94% for cerBb2.The discordance between full section and tissue microarrays was studied using Two  Related  Samples tests and the result had no statistical significance（Pgt;0.05） . ConclusionTissue microarays with 1.6mm in diameter tissue core made by simple method can represent full tissue section on immunohistochemistry study.We believe our method of tissue microarray is a reliable and valid 　　Key words：Tissue microarrays；Making method；Oestrogen receptor；Progesterone receptor;cerBb2 receptor；Immunohistochemistry 　　0引言 　　组织芯片(tissue chip)又称组织微阵列（tissue microarray TMA）。它是将数十至上千个小组织按照设计，整齐地排放在一张载玻片上制