分子生物学 12 Gene manipulation.pptVIP

Section G:  Gene manipulation 1 An overview of DNA cloning Some conception need to know Some useful technology important to gene manipulation Enzymes for DNA cloning Vectors Conception DNA cloning is to place a relatively short fragment of a genome, which might contain the gene or other sequence of interest, in an autonomously replicating piece of DNA, known as a vector, forming recombinant DNA, which can be replicated independently of the original genome, and normally in other host species altogether. Propagation of the host organism containing the recombinant DNA forms a set of g