丹参对小鼠黑色素瘤细胞株B16BL6侵袭和转移能力影响.docVIP

丹参对小鼠黑色素瘤细胞株B16-BL6侵袭和转移能力的影响 作者：徐建亚,顾勤,夏卫军,程罗根 【摘要】 目的 研究活血化瘀中药丹参对小鼠黑色素瘤高转移细胞株B16-BL6生长、粘附、侵袭、运动、转移能力的影响,并初步探讨其作用机制。方法 采用MTT法测定丹参对B16-BL6细胞增殖能力的影响；采用细胞粘附实验、重组基底膜侵袭实验、趋化性运动能力实验以及小鼠黑色素瘤自发性转移模型,观察丹参对B16-BL6细胞生长、粘附、侵袭、运动及转移能力的影响。结果 丹参水提液对B16-BL6细胞的增殖具有双向调节作用；能显著促进B16-BL6细胞与基底膜成分纤维粘连蛋白粘附,显著抑制B16-BL6细胞侵袭基底膜能力及趋化性运动能力；能显著减小肺转移结节体积,但对结节数目没有明显作用。结论 丹参提取液能够显著减轻小鼠黑色素瘤高转移细胞株B16-BL6的肺转移程度,可能与其能够抑制B16-BL6细胞对基底膜的侵袭能力以及降低B16-BL6的运动能力有关。 【关键词】 丹参；黑色素瘤细胞株B16-BL6 　　Abstract：Objective To study the effect of Radix Salviae Miltiorrhizae (RSM), a kind of Chinese Traditional Medicine which can dissolve stasis by activating blood circulation, on proliferation, invasion, adhesion, migration and metastasis of B16-BL6 metastatic mouse melanoma cells and discuss its functional mechanism. Methods The proliferation, adhesion, invasion and migration capacity of B16-BL6 metastatic cells was evaluated by MTT assay, adhesion assay and reconstituted basement membrane invasion and migration assay in vitro respectively. Mouse spontaneous melanoma model was used to study the effect of RSM on metastasis in vivo. Results The extract of RSM bidirectionally adjusted the multiplication of B16-BL6 cells, promoted prominently the adhesion of B16-BL6 to Laminin, inhibited significantly B16-BL6 invading reconstituted basement membrane and the migration of B16-BL6. In the mouse spontaneous melanoma model, it suppressed significantly the volume of lung metastatic nodes but had little effect on the number of lung metastatic nodes. Conclusion The extract of RSM can alleviate the degree of the metastasis of B16-BL6 metastatic mouse melanoma cells, which may be related with inhibiting the B16-BL6 cells invading the extracellular matrix and reducing the migration of B16-BL6 cells. Key words：Radix Salviae Miltiorrhizae (RSM)；B16-BL6 mouse melanoma 　　侵袭与转移是恶性肿瘤最显著的生物学特征,是导致癌症患者临床死亡的主要原因。因此,阻止肿瘤细胞的侵袭和转移是恶性肿瘤治疗成败的关键因素。丹参为唇形科植物丹参(Salvia miltiorrhiza Bge.)的干燥根及根茎,其味苦、微寒,入心、心包及肝经,具有活血通络、祛瘀止痛、凉血消痈等功效,可用于月经不调、经闭痛经、癥瘕积聚、胸腹