不同食物对nafld影响及大鼠肝脏中sirt1及其下游因子表达-effects of different foods on na fld and expression of sir t1 and its downstream factors in rat liver.docxVIP

SREBP-1c and PPARa.Results: The liver histology appeared normal in NC and CR group. High calorie diet treatment induced rat liver pathology similar to clinical NAFLD, however, HFa group rats had a significantly microvesicular steatosis, whereas HFr group rats had a significantly macrovesicular steatosis. The expression of SIRT1 and PGC-1a were increased in group CR, whereas they were decreased in group HFr and HFa. The expression of PPARa in group HFa and HFr were decreased. Inversely, the expression of SREBP-1c in group HFa and HFr were increased. Conclusion: High Fat or fructose diet induced rat liver pathology similar to clinical NAFLD. The mechanism may be that high calorie intake represses the expression of SIRT1, promoting the expression of SREBP-1c which required for fatty acid synthesis, and depressing the expression of PPARα which is a key modulator of fatty acid β-oxidation. Although HFa and HFr diet all lead to hepatocyte steatosis, in HFa rats, hepatocyte steatosis is mainly caused by exogenous fat acid accumulation, whereas in HFr rats it is caused by endogenous fat acid accumulation.Key words：high fat diet;high frcose diet;calorie restriction;SIRT1;steatosis缩 略 词 简 表缩略符号英文名中文名ACCacetyl CoA Carboxylase乙酰辅酶 A 羧化酶ACLATP-citrate lyaseATP 柠檬酸裂解酶ACOAcetyl CoA oxidase乙酰辅酶 A 氧化酶AcrAcrylamide丙烯酰胺APammonium persulfate过硫酸铵BisN,N,-MethylenebisacrylamideN,N,-亚甲双丙烯酰胺BSABovine Serum Albumin牛血清白蛋白CPT-1carnitine palmitoyltransferase肉毒碱棕榈酰转移酶 ICRCalorie restriction能量限制cDNACircular deoxyribonucleic acid环状 DNADNADeoxyribonucleic acid脱氧核糖核酸DTTDithiothreitol二硫苏糖醇EBEthidium Bromide溴化乙锭EDTAEthylenediaminotetra-acetic acid乙二胺四乙酸二钠FINSfasting serum insulin空腹胰岛素FASfatty acid synthase脂肪酸合酶FPGfasting plasma glucose空腹血糖G-6-Paseglucose-6-phosphatase葡萄糖-6-磷酸酶HFaHigh fat diet高脂饮食HFrHigh fructose diet高糖饮食MCDmalonyl-CoA decarboxylase丙二酸单酰辅酶 A 脱羧酶minminute分钟HMG-CoASMitochondrial 3-hydroxy-3-methylglutarylCoA synthase,羟甲基戊二酸单酰辅酶 A合成酶mRNAMessage ribonucleic Acid信使核糖核酸NA