海洋生物附着过程与防污评价技术的研究——利用不同检测技术研究三种细菌的附着-study on the attachment process of marine organisms and anti-fouling evaluation techniques - using different detection techniques to study the attachment of three kinds of bacteria.docxVIP

上海海洋大上海海洋大学硕士学位论文III可逆附着阶段后细菌在材料表面的附着逐渐增多并最终形成成熟的生物膜。第四，从附着基体材料、污损微生物及附着环境三个方面展开讨论，研究了 微生物在材料表面附着的主要影响因素，并发现材料种类、材料的润湿性及材料表面粗糙度均会影响微生物在材料表面的附着量。实验得出以下结论：（1）微生物在不同材料表面的附着量具有以下规律：316L 不锈钢 PVC 玻璃及有机硅材 料；且亲水材料较疏水材料表现出更大的微生物附着量；微生物更倾向于附着在 粗糙度较大的表面，但并非随着粗糙度的增加而持续增加，当材料表面粗糙度降 低至纳米级甚至更低时，附着量随着粗糙度的降低反而增加。（2）对于不同的微 生物而言，由于微生物鞭毛数目及胞外分泌物不同，其附着量也表现出差异， S.oneidensis MR-1 和 P.aeruginosa PAO1 菌株的附着量均大于 E.coli JM109。（3） 不同的环境因素对微生物附着影响表现为：盐度和碳源供给对微生物附着的影响 因微生物的种类不同而异，中性环境和 30℃的温度条件更有利于微生物的附着。 通过以上研究，本文定量描述了微生物在材料表面的附着过程，研究了微生物 附着的主要影响因素，并对微生物附着的检测技术进行了简要评价。可以为研究绿色防污技术提供一定的理论基础。关键词：微生物污损，细菌附着，荧光显微镜技术，附着量IIIIStudy on Attached Process of Marine Organisms and Antifouling Evaluation Technology——Research on attachment of three different bacteria using different detection techniquesABSTRACTWith the blue sea economic strategy carry out, marine industry continue to expand, and several marine vessels and facilities come into being, the scale of marine military, transport and mariculture also expand. But ocean is a complex environment, let marine equipment suffer serious biofouling, then biofouling cause marine corrosion, block the fishing nets and make the meter of marine vessels out of function, then seriously hinder the development of marine economy, which gradually drawn worldwide attention. So explore marine organism’s attachment process and its influencing factors have important practical significance for biofouling research and its solution.In this paper, fluorescence microscope was used for observing the attachment situation of 3 kinds of bacteria on 4 different materials, and the influence of material and environmental on microbial attachment also be examined. The research contents and results were as follows:Firstly, summarized the research status of biofouling at present stage, briefly described the mainly evaluation techniques of microbial attachment, and briefly introduced the characteristics of fluorescence microscopy, atomic force microscope, scanning electron microscopy, m