Biomedical Sensors - Neural Engineering Lab - George ….ppt

Biomedical Sensors Announcements On Wednesday we continue the two groups (1:30pm and 3pm). You are responsible for running the experiments! Webct has a suggested format for lab reports. Sensor is a Transducer:What is a transducer? Biomedical sensors: examples and applications Temperature Accelerometers Pressure sensors Chemical Biochemical Resistance _____________ _____________ _____________ Galvanic skin test Glucose detection Heart rate Vital signs Cochlear implants Retinal implant Cortical implant Health monitoring _____________ _____________ Performance Characteristics 1/3 Performance 2/3 Performance Characteristics 3/3 Chemical Sensors (Biosensors) Biosensing Principles Electrochemical Potentiometric Amperometric FET based Conductometric Optical Piezoelectric Thermal Biosensing Principles Electrochemical Sensors pO2: Historical background Bare metal electrodes: in use since 1923 Davies Brink (1943)1: the recessed electrode Clark (1953 and 1956)2: membrane covered cathode and anode next to each other Biomed-Applications Brain – ischemia, epilepsy Heart – anoxia, hypoxia, drug effects Muscle – contraction coupling, K+ channels Tumors – rate of success of PDT* Eye – cornea, free radicals Others: skin, blood, nerves, liver, etc. In vivo measurements, myocardium In vitro measurements, myocardium Fundamentals of membrane-covered polarographic oxygen electrodes Simplified model for an metabolic active tissue Fabricated device Objective: metabolic activity monitoring in HL-1 cultures. Modified Clark-type oxygen sensorProcessing sequence1 (brief) (layers 1-4): substrate, oxidation, metal, passivation. (layer 5): Nafion:PVP. Nafion 5% in alcohol is mixed to PVP (4:1). Nafion:PVP is deposited on the arrays, and cured at 140oC for 20h. (layer 6): PTFE deposition. Oxygen plasma for 5min (1Torr, 450sccm O2, 500W) Pulsed plasma for 90min (1.3Torr, 100sccm HFPO, 2.3sccm Ar, 0.7W/cm3 peak) (layer 7): encapsulation: epoxy or PDMS. Either epoxy (Supreme 42HT/