毕业设计（论文）-高液相色谱法对样品的分离与纯化.docVIP

高效液相色谱法对样品A的分离与纯化目 录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc483236022摘要 PAGEREF _Toc483236022 \h IHYPERLINK \l _Toc483236024Abstract PAGEREF _Toc483236024 \h IIHYPERLINK \l _Toc483236025引 言 PAGEREF _Toc483236025 \h 1HYPERLINK \l _Toc483236026第1章 绪论 PAGEREF _Toc483236026 \h 2HYPERLINK \l _Toc4832360271.1高效液相色谱简介 PAGEREF _Toc483236027 \h 2HYPERLINK \l _Toc4832360281.1.1正相色谱和反相色谱 PAGEREF _Toc483236028 \h 2HYPERLINK \l _Toc4832360291.1.2制备色谱的概况 PAGEREF _Toc483236029 \h 3HYPERLINK \l _Toc4832360301.2高效液相色谱柱选择与使用 PAGEREF _Toc483236030 \h 6HYPERLINK \l _Toc4832360311.2.1色谱分离模式的选择 PAGEREF _Toc483236031 \h 6HYPERLINK \l _Toc4832360321.2.2制备型高效液相色谱柱 PAGEREF _Toc483236032 \h 7HYPERLINK \l _Toc483236033第2章 样品中杂质的分离与纯化 PAGEREF _Toc483236033 \h 9HYPERLINK \l _Toc4832360342.1实验仪器 PAGEREF _Toc483236034 \h 9HYPERLINK \l _Toc4832360352.2实验试剂 PAGEREF _Toc483236035 \h 9HYPERLINK \l _Toc4832360362.3实验步骤 PAGEREF _Toc483236036 \h 9HYPERLINK \l _Toc483236037第3章 结果与讨论 PAGEREF _Toc483236037 \h 18HYPERLINK \l _Toc483236038结 论 PAGEREF _Toc483236038 \h 19HYPERLINK \l _Toc483236038致 谢 20HYPERLINK \l _Toc483236039参考文献 PAGEREF _Toc483236039 \h 21 PAGE \* MERGEFORMAT20高效液相色谱法对样品A的分离与纯化摘要：高效液相色谱作为一种重要的分析方法，广泛的应用于化学和生物化学中、医药品、化学、环保、生命科学和食品工业的研究上。本论文采用反相高效制备液相色谱对样品A进行了分离，纯化和制备。在样品的制备过程中，对流动相、洗脱梯度、进样量、色谱柱的种类等制备条件进行了优化。优化出制备条件后，使用制备型高效液相色谱对样品进行分离纯化，收集大量样品目标峰的馏分，进行减压蒸馏和冷冻干燥后，得到干燥的样品。经LC-MS检测，其纯度仅有89.733%。通过对液相色谱分离条件的再次优化，经过多次的制备分离，对样品进行富集。经LC-MS检测，最终纯度达到了100%。该方法操作简单，方便，安全性强，污染性小，检测限低。关键词: 高效液相色谱 反相 分离 制备Separation and Purification of Compound A by High Performance Liquid ChromatographyAbstract: High performance liquid chromatography as an important analytical method, widely used in chemical and biochemical, medicine, chemical, environmental protection, life sciences, and food industry research. The samples were separated, purified and prepared by reversed phase high performance liquid chromatography. In the preparation of the sample, the preparation method was screened, including