卵巢黄素化颗粒细胞与胚胎质量和妊娠结局关系探讨.docVIP

卵巢黄素化颗粒细胞与胚胎质量和妊娠结局关系探讨 　　【摘要】目的：探讨在辅助生殖技术中卵巢黄素化颗粒细胞与胚胎质量和妊娠结局的关系。方法：回顾性分析2012年1月至2013年5月因输卵管性因素不育到十堰市人民医院生殖医学中心行IVF-ET的患者，选取全部卵巢黄素化颗粒细胞的110例患者作为黄素化颗粒细胞组，随机选择同期颗粒细胞形态无异常的335例患者作为颗粒细胞形态正常组，比较两组间受精、早期胚胎发育、囊胚形成及妊娠结局的差异。结果：颗粒细胞形态正常组与黄素化颗粒细胞组之间的受精率、优质胚胎率、可利用胚胎率及囊胚形成率差异均有统计学意义（P0.05）。结论：卵巢黄素化颗粒细胞可能影响其受精、胚胎发育及囊胚的形成。 　　【关键词】辅助生殖技术；胚胎；颗粒细胞 　　Relationship between ovarian luteinization granulosa cell， embryo quality and IVF-ET pregnancy outcomeZHANG Xin， DENG Kai， LUO Qinbing， XU Hongyi， ZHANG Changjun△. Center for Reproduction Medicine， Peoples Hospital Affiliated to Hubei University of Medicine， Shiyan 442000， China 　　【Abstract】Objectives： To investigate the relationship between ovarian luteinization granulosa cells， embryo quality and regnancy outcome in assisted reproductive technology. Method： Research objects were selected through retrospective analysis from the IVF-ET patients visiting Center for Reproductive Medicine of Peoples Hospital Affiliated to Hubei University of Medicine from January 2012 to May 2013 due to oviduct factors. 110 patients with ovarian luteinization granulosa cells were regarded as group of luteinization granulosa cells， while 335 patients with no abnormal particle morphology were randomly selected as the normal group. The fertilization， early embryonic development， blastocyst formation and pregnancy outcome of the groups were compared. Results： The differences of fertilization rate， high quality embryo rate， available embryo rate and blastocyst formation rate between the 2 groups were statistically significant （P0.05）. Conclusion： Ovarian luteinization granulosa cells may affect the fertilization， embryo development and blastocyst formation. 　　【Key words】ART； Embryo； Granulosa cells 　　【中图分类号】R711.75【文献标志码】A 　　随着辅助生殖技术的快速发展，控制性超排方案和监测的不断完善，卵裂期胚胎移植或囊胚移植后的妊娠率显著提高，但仍然有40%的患者移植术后未妊娠，其原因和机制尚需进一步研究。如何提高体外受精-胚胎移植（IVF-ET）中的胚胎着床率和妊娠率，一直是胚胎学家和临床医师的追求。 　　在控制超排卵（COH）中，注射人绒毛膜促性腺激素（HCG）后，血清孕酮（P）水平过早的升高，同时在黄体生成素（LH）的作用下伴随颗粒细胞（granulosa cell，GC）过早向黄体细胞分化