Cytocompatibility of chitosan―based thermosensitive hydrogel to human perioontal ligament cell.docVIP
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Cytocompatibility of chitosan―based thermosensitive hydrogel to human perioontal ligament cell
Cytocompatibility of chitosan―based thermosensitive hydrogel to human periodontal ligament cell
【Abstract】Objective: To investigate the effect of thermosensitive chitosan/β-glycerophosphate (CS/β-GP) hydrogel on proliferation of human periodontal ligament cells (HPDLCs). Methods: CS/β-GP were prepared into a thermosensitive hydrogel and its three-dimensional structure was observed under electron microscope. HPDLCs harvested and cultured in vitro were co-cultured with the thermosensitive CS/β-GP hydrogel. Growth of the cells in the hydrogel was observed with HE staining, and the effect of the extract on proliferation of HPDLCs was examined by CCK-8 assay. Results: Observations of SEM and HE staining showed that the thermosensitive CS/β-GP hydrogel was large in pore size and appropriate for cell growth. Different levels of CS/α,β-GP extracts could promote proliferation of HPDLCs. Conclusion: Thermosensitive CS/β-GP hydrogel can promote proliferation of HPDLCs and be a good carrier for periodontal tissue engineering because of its thermosensitivity.
中国论文网 /6/viewhtm
【key words 】 CS/β-GP PDLCs CCK8
【CLC】R722.12 【Document code】B【Article number】1004-4949(2015)03-0516-02
High incidence of periodontal disease may result in destruction of periodontal supporting tissues. PDLC is of multiple differentiation potential, and plays a key role in periodontal tissue regeneration. Modern periodontal tissue engineering is expected to solve this problem, by which a certain amount of PDLCs are inoculated into an appropriate scaffold and form a cell-scaffold complex for co-culture. HPDLCs harvested and cultured in vitro are to be co-cultured with CS/β-GP hydrogel to investigate the biocompatibility of CS/β-GP with PDLCs.
Main materials and equipment FBS, α-MEM, CS , β-GP, Cell Counting Kit-8 , HE Staining Kit , magnetic stirrer , scanning electron microscope, CO2 Incubator , Thermo Scientific Microplate Reader.
Methods
Preparation and property detection o
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