HPLC同时测定三汤水提物中没食子酸、柯里拉京及鞣花酸含量.docVIP

HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量 　　摘要：目的 建立HPLC同时测定三果汤水提物中没食子酸、柯里拉京及鞣花酸含量的方法。方法 采用Agilent ZORBAX SB-C18色谱柱（4.6 mm×250 mm，5 ?m），流动相为乙腈-0.1%磷酸水溶液，梯度洗脱，检测波长为260 nm，柱温30 ℃，流速为1.0 mL/min。结果 没食子酸、柯里拉京及鞣花酸3种成分有较好的基线分离，且峰形较好，分别在0.174～2.61 ?g、0.04～0.60 ?g、0.052～0.78 ?g范围内线性关系良好；平均加样回收率分别为99.98%、99.81%、100.12%，RSD均小于2.0%。结论 本方法快速、准确、重复性好，可为三果汤水提物的质量控制提供依据。 中国论文网 /1/viewhtm　　关键词：三果汤水提物；高效液相色谱法；没食子酸；柯里拉京；鞣花酸 　　DOI：10.3969/j.issn.1005-5304.2017.09. 　　中图分类号：R284.1 文献标识码：A 文章编号：1005-5304（2017）09- 　　Content Determination of Gallic Acid， Corilagin and Ellagic Acid in Aqueous Extract from Sanguo Decoction by HPLC LI Bin1， LI Xin2， FAN Yuan1 （1. School of Chinese Medicine， Yunnan University of Chinese Medicine， Kunming 650200， China； 2. Hunan Provincial Key Laboratory of Diagnostics in Chinese Medicine， Hunan University of Chinese Medicine， Changsha 410007， China） 　　Abstract： Objective To establish an HPLC method for the simultaneous determination of contents of gallic acid， corilagin and ellagic acid in aqueous extract from Sanguo Decoction. Methods The analysis was carried out on an Agilent C18 column （4.6 mm × 250 mm， 5 ?m）， and the mobile phase was composed of acetonitrile and 0.1% phosphoric acid aqueous with gradient elution. The detection wavelength was 260 nm. The flow rate was 1.0 mL/min at column temperature of 30 ℃. Results Gallic acid， corilagin and ellagic acid were completely separated and the peak shape was good. It showed a good linearity in the concentration range of 0.174?C2.61 ?g， 0.04?C0.60 ?g， and 0.052?C0.78 ?g， respectively. The average recoveries of gallic acid， corilagin and ellagic acid were 99.98%， 99.81% and 100.12%， respectively. The RSD were less than 2.0%. Conclusion This method is rapid， accurate and repeatable， which can provide references for the quality control of Sanguo Decoction. 　　Key words： aqueous extract from Sanguo Decoction； HPLC； gallic acid； corilagin； ellagic acid 　　三果??由诃子、毛诃子、余甘子3味药组成，为藏医学的重要基础方，具有清热、调和气血、化解坏血功效[1]。《四部医典》称其“主治瘟疫