CMIA法在梅毒血清学检测中应用评价.docVIP

CMIA法在梅毒血清学检测中应用评价 　　[摘要] 目的 探讨化学发光微粒子免疫分析（CMIA）法在TP感染血清学检测中的临床应用。方法 收集该院2013年7月―2013年11月门诊及住院部经CMIA法检测疑似梅毒阳性（S/CO≥1.0）标本270例进行临床研究，并对阳性标本采用TPPA和TP-ELISA法进行检测。结果 270例CMIA法阳性标本中，TPPA阳性检出率81.85%（221/270），TP-ELISA阳性检出率83.33%（225/270）。TPPA、TP-ELISA与CMIA对比有差异有统计学意义，高、中浓度对比分析差异无统计学意义，低浓度对比分析差异有统计学意义。结论 CMIA法较TPPA和TP-ELISA法具有更高的特异性及敏感性，用于梅毒螺旋体特异性抗体筛查有助于提高准确性与阳性检出率。 　　[关键词] 梅毒螺旋体；化学发光微粒子免疫分析；梅毒螺旋体明胶凝集试验 　　[中图分类号] R446.1 [文献标识码] A [文章编号] 1674-0742（2014）07（a）-0176-02 　　[Abstract] Objective To investigate the clinical application of chemiluminescent microparticle immunoassay （CMIA） in serological detection of TP infection. Methods 270 cases of specimens suspected with syphilis positive （S/CO≥1.0） detected by CMIA method in the Outpatient Department and Inpatient Department of our hospital from July， 2013 to November， 2013 were collected for the clinical study. And the positive specimens were detected by TPPA and TP-ELISA method. Results Of the 270 cases of positive specimens detected by CMIA method， the positive detection rate of TPPA was 81.85%（221/270）， and that of TP-ELISA was 83.33%（225/270）. The difference between TPPA， TP-ELISA and CMIA was statistically significant by comparison， high concentrations of comparative analysis showed no statistically significant difference while low concentrations of comparative analysis showed statistically significant difference. Conclusion Compared with TPPA method and TP-ELISA method， CMIA method has higher sensitivity and specificity for screening of treponema pallidum antibodies， which helps to improve the accuracy and the positive rate of detection. 　　[Key words] Treponema pallidum； Chemiluminescence microparticle immunoassay； Treponema pallidum particle agglutination test 　　梅毒螺旋体为梅毒的病原体，梅毒是一种具有较强的传染性性传播疾病。世界卫生组织（WHO）报告，全世界每年新增1200万梅毒感染者[1]。目前，梅??螺旋体抗体血清学检测以TPPA及TP-ELISA法居多，而CMIA法应用较晚。为探究CMIA法在梅毒血清学检测中的应用评价，该研究于2013年4月起，开始着手实验相关策划准备，历时半年，与研究团队收集2013年7月―2013年11月来该院门诊经CMIA法检测270例疑似梅毒阳性（S/CO≥1.0）标本，分别对CMIA法检测阳性标本进行TPPA及TP-ELISA对照