细胞机理.pptVIP

[Target] (1) Understand the basic principles of PEG-induced cell fusion (2) Grasp the techniques of basic PEG-induced cell fusion [Principles] We use the chick red blood cell which has an obvious nuclear structure. PEG (polyethylene glycol), is a compound of a number of different molecular weight polymer. It can bind with water molecules by hydrogen bond, which causes free water to disappear in high concentration (50%) of PEG solution, leading to cell dehydration and plasma membrane structure changes, then makes cell fusion. PEG can promote cell fusion effectively only in high concentration