Nanodrop应用-分光光度法对工业染色剂的定量分析.docxVIP

NanoDrop 2000/2000c应用分光光度法对工业染色剂的定量分析Richard W. Beringer and Andrew F. Page Thermo Fisher Scientific, Wilmington, DE Introduction 介绍Quality control of industrial dye additives is imperative to ensure the reproducibility of dye color and appearance in the colorant’s final application. The Thermo Scientific NanoDrop 2000 UV-Vis Spectrophotometer offers a rapid, fully customizable method for checking batch quality for proper pigment concentration prior to distribution to the product’s end user. The concentrations of most aqueous dyes and pigments can be assessed using the full ultraviolet and visible spectrum capabilities of the NanoDropTM 2000. 为了确认染色的重复性和着色剂在最终应用中的表现，工业染料添加剂的质量控制非常必要。赛默飞世尔的NanoDrop 2000紫外可见分光光度计提供一个快速、顾客自定义的方法，在产品出售给最终用户之前，预先成批检测的染料在一个合理的浓度范围。大多数水溶性染料和天然色素的浓度可以用覆盖紫外和可见区域的NanoDrop 2000紫外分光光度计进行测试。The NanoDrop 2000 utilizes auto-ranging pathlengths to quantify dye samples across a much broader concentration range than is possible using a conventional cuvette-based spectrophotometer. By optimizing the pathlength based on dye absorbance (ranging from 1.0 mm to 0.05 mm) the NanoDrop 2000 can accurately measure the absorbance of a sample across a concentration range nearly 200 fold greater than that of a cuvette-based system. In this study, the visible absorbance spectrum of uranine, the disodium salt of fluorescein, was assessed using a NanoDrop 2000 Spectrophotometer across a broad concentration range. Uranine is commonly used in oil and gas industries to detect leaks in containment vessels and pipelines. It is also used as a colorant in military, medical, and cosmetic applications. Both linear dynamic range and precision (reproducibility) were assessed. 相比传统的比色皿紫外分光光度法，NanoDrop 2000紫外可见分光光度计在定量染料样品浓度时，通过利用自动光程控制，能得到更宽的浓度范围。通过根据染料的吸光度来优化光程（1.0mm至0.05mm），NanoDrop 2000可以正确测定样品吸光度的浓度范围比常规比色皿分析浓度范围宽200倍。这次研究中，二钠盐荧光素的可见吸光谱图，就是用NanoDrop 2000紫外分光光度计在一个很宽的浓度范围进行分析。荧光素钠是在油和气体工业中常用检测项目用以确认容器或管道中是否发生泄漏。它作为一种着色剂还用于军事、药物和化妆品应用邻域。线性范围和精密度（重复性）给出测试。Experimental